Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Comparing the Expression Levels of Mrna for Mmp-7 in Gastric Mucosa of Patients With H. Pylori Infection and Uninfected Patients



Sadeghiani M1 ; Shahi H1 ; Bagheri N2 ; Reiisi S3 ; Rahimian G4 ; Rashidii R4 ; Shafigheardestani M4 ; Chaleshtori MH5 ; Rafieiankopaei M6 ; Ramezani G7 ; Fatollahi F7 ; Shahverdi E7 ; Shirzad H5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
  2. 2. School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Genetics, Faculty of Basic Sciences, University of Shahrekord, Shahrekord, Iran
  4. 4. Department of Gastroenterology, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
  5. 5. Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
  6. 6. Medicinal Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
  7. 7. Hajar Hospital, Shahrekord University of Medical Sciences, Shahrekord, Iran

Source: Journal of Mazandaran University of Medical Sciences Published:2016

Abstract

Background and purpose: The expression of growth factors, proteolytic enzymes, fibrogenic factors, and cytokines are altered in Helicobacter pylori (H. pylori) infected gastric mucosa. Matrix metalloproteinases (MMP) are a family of zinc-dependent homologous enzymes digesting most of the components of the extracellular matrix and basement membrane and are involved in remodeling and functioning of the biological processes. The purpose of this study was to compare gene expression of matrix metalloproteinase-7 (MMP-7) in patients with H. pylori-infected and uninfected individuals with gastrointestinal diseases. Materials and methods: This study was conducted in 50 H. pylori-negative patients and 50 H. pylori-positive patients being admitted to Shahrekord Hajar Hospital due to gastrointestinal diseases in 2014. The participants’ demographic information was collected and sampling was done. First DNA was extracted, and then PCR was performed to check for the presence of 16sRNA and UreC. The RNA from each sample was also extracted and cDNA was prepared. Afterwards, the expression of MMP-7 was measured by real time-PCR using specific primers and probes. Results: MMP-7 mRNA expression was significantly higher in biopsies of H. pylori-infected patients compared to that in H. pylori-uninfected patients (P<0.0001). Conclusion: Increased expression of MMP-7 can be effective in inflammatory response and development of the disease. It could be used as a key marker for early diagnosis of gastrointestinal diseases and gastric cancer. © 2016, Mazandaran University of Medical Sciences. Engineering. All rights reserved.
Related Docs
View other Related Docs
1. Cag Pathogenicity Island-Dependent Upregulation of Matrix Metalloproteinase-7 in Infected Patients With Helicobacter Pylori, Journal of Immunoassay and Immunochemistry (2017)
2. Role of Regulatory T-Cells in Different Clinical Expressions of Helicobacter Pylori Infection, Archives of Medical Research (2016)
3. Clinical Relevance of Helicobacter Pylori Virulence Factors in Iranian Patients With Gastrointestinal Diseases, Microbial Pathogenesis (2016)
4. The Effect of Tlr4 Asp299gly Polymorphism on Il-6 and Il -18 Expression in H.Pylori Infected Patients, Journal of Kerman University of Medical Sciences (2016)
5. Altered Th17 Cytokine Expression in Helicobacter Pylori Patients With Tlr4 (D299g) Polymorphism, Immunological Investigations (2016)
6. Up-Regulated Th17 Cell Function Is Associated With Increased Peptic Ulcer Disease in Helicobacter Pylori-Infection, Infection# Genetics and Evolution (2018)
7. Correlation Between Mucosal Il-6 Mrna Expression Level and Virulence Factors of Helicobacter Pylori in Iranian Adult Patients With Chronic Gastritis, Jundishapur Journal of Microbiology (2015)
8. Comparative Immune Response in Children and Adults With H. Pylori Infection, Journal of Immunology Research (2015)
Experts (# of related papers)
Sayed Alireza Razavi (5)
Other Related Docs
9. Mucosal Interleukin-21 Mrna Expression Level Is High in Patients With Helicobacter Pylori and Is Associated With the Severity of Gastritis, Central European Journal of Immunology (2015)
10. Downregulated Regulatory T Cell Function Is Associated With Increased Peptic Ulcer in Helicobacter Pylori-Infection, Microbial Pathogenesis (2017)
11. Inhibition of Th1 and Th17 Cells by Medicinal Plants and Their Derivatives: A Systematic Review, Phytotherapy Research (2017)
12. The Biological Functions of Il-17 in Different Clinical Expressions of Helicobacter Pylori-Infection, Microbial Pathogenesis (2015)
13. The Role of Th1 and Th17 Cells in Glomerulonephritis, Journal of Nephropathology (2015)
14. New Insight to Il-23/Il-17 Axis in Iranian Infected Adult Patients With Gastritis : Effects of Genes Polymorphisms on Expression of Cytokines, Acta Gastro-Enterologica Belgica (2015)
15. Frequency of Virulence Factors in Helicobacter Pylori-Infected Patients With Gastritis, Microbial Pathogenesis (2015)
16. Frequency of Helicobacter Pylori Hopqi, Hopqii and Saba Genes Among Iranian Patients With Gastroduodenal Diseases, Jundishapur Journal of Microbiology (2018)
17. T-Bet+ Cells Polarization in Patients Infected With Helicobacter Pylori Increase the Risk of Peptic Ulcer Development, Archives of Medical Research (2019)