Evaluation of Colonic Anastomosis Healing Using Hybrid Nanosheets Containing Molybdenum Disulfide (Mos2) Scaffold of Human Placental Amniotic Membrane and Polycaprolactone (Pcl) in Rat Animal Model Publisher Pubmed



Ebrahim Soltani Z^{1, 2} ; Elahi M^{1, 2} ; Tashakgolroudbari H^{1, 3} ; Nazari H⁴ ; Badripour A³ ; Heiranitabasi A¹ ; Akbari Asbagh R^{1, 3} ; Dabbagh Ohadi MA^{1, 3} ; Shabani M¹ ; Sarzaeim M^{3, 5} ; Behboudi B² ; Keramati MR² ; Kazemeini A² ; Ahmadi Tafti SM² Show Affiliations
Source: Naunyn-Schmiedeberg's Archives of Pharmacology Published:2023

Abstract

Anastomosis is a standard technique following different conditions such as obstruction, tumor, and trauma. Obstruction, adhesion, or anastomosis leakage can be some of its complications. To improve healing and prevent postoperative complications, we design a hybrid scaffold containing acellular human amniotic membranes and polycaprolactone-molybdenum disulfide nanosheets for colon anastomosis. The animal model of colocolonic anastomosis was performed on two groups of rats: control and scaffold. The hybrid scaffold was warped around the anastomosis site in the scaffold group. Samples from the anastomosis site were resected on the third and seventh postoperative days for histopathological and molecular assessments. Histopathologic score and burst pressure had shown significant improvement in the scaffold group. No mortality and anastomosis leakage was reported in the scaffold group. In addition, inflammatory markers were significantly decreased, while anti-inflammatory cytokines were increased in the scaffold group. The result indicates that our hybrid scaffold is a proper choice for colorectal anastomosis repair by declining postoperative complications and accelerating healing. Graphical Abstract: [Figure not available: see fulltext.] © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.