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Method Validation for Simultaneous Determination of Macrolides, Quinolones, and Sulfonamides Antibiotics in Iranian Yogurt Samples by Spe Clean-Up and Uhplc-Ms/Ms Publisher



Kheirandish S1 ; Goudarzi S2 ; Amirahmadi M2, 3 ; Ghareghani S1 ; Ghafari H1 ; Daraei B4 ; Ghazikhansari M1
Authors
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Authors Affiliations
  1. 1. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Food and Drug Reference Control Laboratories, Food and Drug Organization, MOH & ME, Tehran, Iran
  3. 3. Food and Drug Laboratory Research Center, Food and Drug Organization, MOH & ME, Tehran, Iran
  4. 4. Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Science, Tehran, Iran

Source: Microchemical Journal Published:2023


Abstract

The transmission of antibiotic residues through the food chain to humans is one of the main concerns of human health due to the emergence of various adverse effects, such as antibiotic resistance. The primary source of antibiotic residues in foods driven from animals is the misuse of antimicrobial drugs in animals. This study aimed to use a selective multi-residues method using ultra-high performance liquid chromatography coupled to tandem mass spectrometry on solid-phase extraction for simultaneous detection and analysis of 25 antibiotics from three different antibiotic groups of quinolones, sulfonamides, and macrolides as well as griseofulvin and trimethoprim in Iranian yogurt samples. The limit of quantification and limit of detection were obtained at 10.0 and 3.3 ng g−1, respectively. The average recovery was in the range of 96–116% for almost all analytes. The inter-day and intra-day precision showed relative standard deviation values lower than 20%. The validated method was applied to determine 25 target analytes of 34 pasteurized and traditional Iranian low-fat yogurt samples, and the results showed no antibiotic contamination. © 2023 Elsevier B.V.
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