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Effect of Transduced Mesenchymal Stem Cells With Il-10 Gene on Control of Allergic Asthma Publisher Pubmed



Hou C1 ; Sun F1 ; Liang Y2 ; Nasab EM3 ; Athari SS4
Authors
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Authors Affiliations
  1. 1. Department of Respiratory and Critical Care Medicine, Henan Provincial Chest Hospital, Henan, Zhengzhou, 450008, China
  2. 2. Kidney transplantation nephropathy diagnosis and treatment center, The 7th People’s Hospital of Zhengzhou, Henan, Zhengzhou City, 450016, China
  3. 3. Department of Cardiology, School of Medicine, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Immunology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

Source: Allergologia et Immunopathologia Published:2023


Abstract

Asthma is an important pulmonary disease associated with T helper lymphocyte (Th)2 dominant immune response, which can initiate allergic and inflammatory reactions. Interleukin (IL)-10 is the main immune suppressor cytokine, and mesenchymal stem cells (MSCs) have an immune-modulatory potential that can be transduced with the expression of the IL-10 gene to control pathophysiology of allergic asthma. Bone marrow’s MSCs were isolated and transduced with the expression vector that contains the expressible IL-10 gene. Then, allergic asthma mouse model was produced and treated with manipulated MSCs. Methacholine challenge test; measurement of IL-4, IL-5, IL-8, IL-13, IL-25, and IL-33; and total and ovalbumin (OVA)-specific immunoglobulin (Ig)E levels were done. Hyperplasia of the goblet cell, secretion of mucus, and peribronchiolar and perivascular eosinophilic inflammation were evaluated in lung pathological sections. IL-25, IL-33, and total IgE levels; AHR; eosinophilic inflammation; hyperplasia of the goblet cell; and secretion of mucus could be controlled in M, MV, and MV-10 groups, and the control in the MV-10 group was strong compared to M and MV groups. MSCs have immune-modulatory capacity that can control allergic asthma pathophysiology, and this effect can be strengthened and reinforced by the expression of IL-10 gene. © 2023 Codon Publications. Published by Codon Publications © Hou C, et al. License: This open access article is licensed under Creative Commons Attribution 4.0 International (CC BY 4.0). http://creativecommons.org/
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