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Evaluation of the in Vitro Biodegradation and Biological Behavior of Poly(Lactic-Co-Glycolic Acid)/Nano-Fluorhydroxyapatite Composite Microsphere-Sintered Scaffold for Bone Tissue Engineering Publisher



Tahriri M1, 2, 3 ; Moztarzadeh F2 ; Tahriri A4 ; Eslami H5 ; Khoshroo K1 ; Jazayeri HE6 ; Tayebi L1, 7
Authors
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Authors Affiliations
  1. 1. School of Dentistry, Marquette University, Milwaukee, WI, United States
  2. 2. Biomaterials Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran
  3. 3. Dental Biomaterials Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Faculty of Management, University of Tehran, Tehran, Iran
  5. 5. Department of Biomedical Engineering, Haeri University of Meybod, Yazd, Iran
  6. 6. School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, United States
  7. 7. Department of Engineering Science, University of Oxford, Oxford, United Kingdom

Source: Journal of Bioactive and Compatible Polymers Published:2018


Abstract

The objective of this research was to study the degradation and biological characteristics of the three-dimensional porous composite scaffold made of poly(lactic-co-glycolic acid)/nano-fluorhydroxyapatite microsphere using sintering method for potential bone tissue engineering. Our previous experimental results demonstrated that poly(lactic-co-glycolic acid)/nano-fluorhydroxyapatite composite scaffold with a ratio of 4:1 sintered at 90ºC for 2 h has the greatest mechanical properties and a proper pore structure for bone repair applications. The weight loss percentage of both poly(lactic-co-glycolic acid)/nano-fluorhydroxyapatite and poly(lactic-co-glycolic acid) scaffolds demonstrated a monotonic trend with increasing degradation time, that is, the incorporation of nano-fluorhydroxyapatite into polymeric scaffold could lead to weight loss in comparison with that of pure poly(lactic-co-glycolic acid). The pH change for composite scaffolds showed that there was a slight decrease until 2 weeks after immersion in simulated body fluid, followed by a significant increase in the pH of simulated body fluid without a scaffold at the end of immersion time. The mechanical properties of composite scaffold were higher than that of poly(lactic-co-glycolic acid) scaffold at total time of incubation in simulated body fluid; however, it should be noted that the incorporation of nano-fluorhydroxyapatite into composite scaffold leads to decline in the relatively significant mechanical strength and modulus during hydrolytic degradation. In addition, MTT assay and alkaline phosphatase activity results defined that a general trend of increasing cell viability was seen for poly(lactic-co-glycolic acid)/nano-fluorhydroxyapatite scaffold sintered by time when compared to control group. Eventually, experimental results exhibited poly(lactic-co-glycolic acid)/nano-fluorhydroxyapatite microsphere-sintered scaffold is a promising scaffold for bone repair. © 2017, © The Author(s) 2017.
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