Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
A Comparison Study on the Behavior of Human Endometrial Stem Cell-Derived Osteoblast Cells on Plga/Ha Nanocomposite Scaffolds Fabricated by Electrospinning and Freeze-Drying Methods Publisher Pubmed



Namini MS1 ; Bayat N2, 3 ; Tajerian R2 ; Ebrahimibarough S2 ; Azami M2 ; Irani S1 ; Jangjoo S4 ; Shirian S5, 6 ; Ai J2, 3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Islamic Azad University, Department of Biology, Science and Research Branch, Tehran, Iran
  2. 2. Tehran University of Medical Sciences, Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran, Iran
  3. 3. Tehran University of Medical Sciences, Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran, Iran
  4. 4. Shiraz University of Medical Sciences, School of Medicine, Shiraz, Iran
  5. 5. Shahrekord University, Department of Pathology, School of Veterinary Medicine, Shahr-e Kord, Iran
  6. 6. Shiraz Molecular Pathology Research Center, Dr Daneshbod Lab Pathology, Shiraz, Iran

Source: Journal of Orthopaedic Surgery and Research Published:2018


Abstract

Background: An engineered tissue structure is an artificial scaffold combined with cells and signaling factors. Among various polymers, the polylactide-co-glycolide/hydroxyapatite (PLGA/HA) has attracted much attention due to their optimal properties. The aim of this study was to study the behavior of human endometrial stem cell (hEnSC)-derived osteoblast cells cultured on PLGA/HA nanocomposite scaffolds. Methods: hEnSCs were isolated and exposed to osteogenic media for 21 days. Differentiated cells were cultured on PLGA/HA synthetic scaffolds. The PLGA/HA-based nanocomposite scaffolds were fabricated using either electrospinning or freeze-drying methods. Behavior of the cells was evaluated a week after seeding hEnSC-derived osteoblast-like cells on these scaffolds. Osteogenesis was investigated in terms of alkaline phosphatase activity, gene expression, immunocytochemistry (ICC), proliferation, and scanning electron microscopy (SEM). Moreover, scaffold properties, such as pore size and morphology of the cells, onto the scaffolds were evaluated using SEM. Furthermore, biocompatibility of these scaffolds was confirmed by 3-(4,5-dimethylthiazoyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The matrix mineralization was proved by alizarin red staining, and the osteogenic media-treated cultures positively expressed osteocalcin and osteopontin markers. Moreover, qRT-PCR results confirmed the positive gene expression of osteopontin and osteonectin in the differentiated osteoblast-like cells. The results of behavior assessment of the cultured cells on electrospinning and freeze-dried scaffolds showed that the behavior of the cultured cells on the freeze-dried PLGA/HA scaffolds was significantly better than the electrospinning PLGA/HA scaffolds. Conclusion: It has been shown that the freeze-dried PLGA/HA nanocomposite scaffolds can appropriately support the attachment and proliferation of the differentiated osteoblast cells and are a suitable candidate for bone tissue engineering. © 2018 The Author(s).
Related Docs
View other Related Docs
1. Investigating the Neuroglial Differentiation Effect of Neuroblastoma Conditioned Medium in Human Endometrial Stem Cells Cultured on 3D Nanofibrous Scaffold, Journal of Biomedical Materials Research - Part A (2015)
2. In Vitro Evaluation of Human Endometrial Stem Cell-Derived Osteoblast-Like Cells’ Behavior on Gelatin/Collagen/Bioglass Nanofibers’ Scaffolds, Journal of Biomedical Materials Research - Part A (2016)
3. Mesenchymal Endometrial Stem/Stromal Cells for Hard Tissue Engineering: A Review of in Vitro and in Vivo Evidence, Regenerative Medicine (2017)
4. Cellular Activity of Wharton's Jelly-Derived Mesenchymal Stem Cells on Electrospun Fibrous and Solvent-Cast Film Scaffolds, Journal of Biomedical Materials Research - Part A (2016)
5. Silk Fibroin/Hydroxyapatite Composites for Bone Tissue Engineering, Biotechnology Advances (2018)
6. Inhibitor of Pi3k/Akt Signaling Pathway Small Molecule Promotes Motor Neuron Differentiation of Human Endometrial Stem Cells Cultured on Electrospun Biocomposite Polycaprolactone/Collagen Scaffolds, Molecular Neurobiology (2017)
7. Silk As a Potential Candidate for Bone Tissue Engineering, Journal of Controlled Release (2015)
8. Evaluation of Motor Neuron-Like Cell Differentiation of Henscs on Biodegradable Plga Nanofiber Scaffolds, Molecular Neurobiology (2015)
Experts (# of related papers)
View all Related Experts
Somayeh Ebrahimi Barough (18)
Mahmoud Azami (4)
Other Related Docs
9. Purmorphamine As a Shh Signaling Activator Small Molecule Promotes Motor Neuron Differentiation of Mesenchymal Stem Cells Cultured on Nanofibrous Pcl Scaffold, Molecular Neurobiology (2017)
10. The Effect of Purmorphamine on Differentiation of Endometrial Stem Cells Into Osteoblast-Like Cells on Collagen/Hydroxyapatite Scaffolds, Artificial Cells# Nanomedicine and Biotechnology (2017)
11. Sustained Release of Valproic Acid Loaded on Chitosan Nanoparticles Within Hybrid of Alginate/Chitosan Hydrogel With/Without Stem Cells in Regeneration of Spinal Cord Injury, Progress in Biomaterials (2023)
12. Osteogenic Differentiation of Follicular Stem Cells on Nano-Saghez Scaffold Containing Bmp2, Journal of Orthopaedic Surgery and Research (2019)
13. Mesenchymal Stem Cells Act As Stimulators of Neurogenesis and Synaptic Function in a Rat Model of Alzheimer's Disease, Heliyon (2021)
14. Osteoconduction of Unrestricted Somatic Stem Cells on an Electrospun Polylactic-Co-Glycolic Acid Scaffold Coated With Nanohydroxyapatite, Cells Tissues Organs (2018)
15. Stem Cell Therapy in Limb Ischemia: State-Of-Art, Perspective, and Possible Impacts of Endometrial-Derived Stem Cells, Frontiers in Cell and Developmental Biology (2022)
16. Endometrial and Menstrual Blood Mesenchymal Stem/Stromal Cells: Biological Properties and Clinical Application, Frontiers in Cell and Developmental Biology (2020)
17. Differentiation of Neural Crest Stem Cells From Nasal Mucosa Into Motor Neuron-Like Cells, Journal of Chemical Neuroanatomy (2018)
18. Bridging Potential of Taurine-Loading Pcl Conduits Transplanted With Henscs on Resected Sciatic Nerves, Regenerative Therapy (2022)
19. Endometrial Stem Cells Differentiation Into Neural Cells by Ly294002 Small Molecule, Koomesh (2016)
20. Osteogenic Potential of Stem Cells-Seeded Bioactive Nanocomposite Scaffolds: A Comparative Study Between Human Mesenchymal Stem Cells Derived From Bone, Umbilical Cord Wharton's Jelly, and Adipose Tissue, Journal of Biomedical Materials Research - Part B Applied Biomaterials (2018)
21. Electrospun Nerve Guide Scaffold of Poly(Ε-Caprolactone)/Collagen/Nanobioglass: An in Vitro Study in Peripheral Nerve Tissue Engineering, Journal of Biomedical Materials Research - Part A (2017)
22. Pcl/Gelatin Nanofibrous Scaffolds With Human Endometrial Stem Cells/Schwann Cells Facilitate Axon Regeneration in Spinal Cord Injury, Journal of Cellular Physiology (2019)
23. Proanthocyanidin As a Crosslinking Agent for Fibrin, Collagen Hydrogels and Their Composites With Decellularized Wharton’S-Jelly-Extract for Tissue Engineering Applications, Journal of Bioactive and Compatible Polymers (2020)
24. Microtubule Stabilizer Epothilone B As a Motor Neuron Differentiation Agent for Human Endometrial Stem Cells, Cell Biology International (2020)
25. The Differentiation of Human Endometrial Stem Cells Into Neuron-Like Cells on Electrospun Pan-Derived Carbon Nanofibers With Random and Aligned Topographies, Molecular Neurobiology (2016)
26. Differentiation of Endometrial Stem Cells Into Motor Neurons by the Use of Purmorphamin Small Molecule, Tehran University Medical Journal (2017)
27. Preparation of Collagen/Polyurethane/Knitted Silk As a Composite Scaffold for Tendon Tissue Engineering, Proceedings of the Institution of Mechanical Engineers# Part H: Journal of Engineering in Medicine (2017)
28. Differentiation of Human Endometrial Stem Cells Into Schwann Cells in Fibrin Hydrogel As 3D Culture, Molecular Neurobiology (2016)
29. Chimeric Self-Assembling Nanofiber Containing Bone Marrow Homing Peptide’S Motif Induces Motor Neuron Recovery in Animal Model of Chronic Spinal Cord Injury; an in Vitro and in Vivo Investigation, Molecular Neurobiology (2016)
30. The Role of Mirnas 340-5P, 92A-3P, and 381-3P in Patients With Endometriosis: A Plasma and Mesenchymal Stem-Like Cell Study, BioMed Research International (2021)
31. Growth Factor-Loaded Nano-Niosomal Gel Formulation and Characterization, AAPS PharmSciTech (2017)
32. Advances in Management of Spinal Cord Injury Using Stem Cell-Derived Extracellular Vesicles: A Review Study, Basic and Clinical Neuroscience (2023)