Determination of Helicobacter Pylori Virulence-Associated Genes in Duodenal Ulcer and Gastric Biopsies Publisher



Saeidi Y¹ ; Pournajaf A² ; Gholami M² ; Hasannejadbibalan M² ; Yaghoubi S³ ; Khodabandeh M⁴ ; Emadi B⁵ ; Ferdosishahandashti E⁶ ; Rajabnia R⁷ Show Affiliations
Source: Medical Journal of the Islamic Republic of Iran Published:2017

Abstract

Background: Helicobacter pylori (H. pylori or Hp) has been strongly associated with the peptic ulcer diseases, chronic gastritis, ulcers, and gastric cancer. Genes associated with pathogenicity have been designated for H. pylori, and some of them appear to be related to more severe clinical consequences of the infection. The present study was conducted to determine cagA, vacA, cagE, iceA1, oipA, and iceA2 genes in H. pylori strains isolated from gastroduodenal patients, who referred to Shariati hospital in Tehran, Iran. Methods: Gastric biopsy specimens were collected during endoscopy from patients, who referred to the Shariati hospital in Tehran, Iran during January and November 2015. After isolation of H. pylori from the biopsy culture, genomic DNA was extracted and subsequently used to identify H. pylori and virulence genes using specific primers. Results: The isolation rate of H. pylori strains was 65.7% (169/257). The frequency of cagA, vacA, cagE, iceA1, oipA, and iceA2 was 143 (% 84.6), 169 (100%), 131 (77.5%), 97 (57.3%), 89 (52.6%), and 72 (42.6%), respectively. Conclusion: In this study, a significant difference was observed between investigated genes and strains isolated from PUD and GC patients (p < 0.05). © Iran University of Medical Sciences.