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Amniotic Membrane Seeded Fetal Fibroblasts As Skin Substitute for Wound Regeneration Publisher Pubmed



Taghiabadi E1, 2 ; Beiki B2 ; Aghdami N1 ; Bajouri A1
Authors

Source: Methods in Molecular Biology Published:2019


Abstract

Various natural and synthetic biomaterials have been applied as skin substitutes for regenerating damaged skin. Here, we describe a straightforward method for fabrication of a tissue-engineered skin substitute by seeding human fetal fibroblasts on acellular human amniotic membrane (HAM). Fetal fibroblasts are achieved from the skin of normal and non-macerated fetus of 11–14 weeks old after spontaneous pregnancy termination. Acellular HAM is obtained by separation of the outer membrane of the chorion and removing its epithelial cells. © Springer Science+Business Media New York 2018.
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