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Amniotic Membrane Seeded Fetal Fibroblasts As Skin Substitute for Wound Regeneration Publisher Pubmed



Taghiabadi E1, 2 ; Beiki B2 ; Aghdami N1 ; Bajouri A1
Authors
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Authors Affiliations
  1. 1. Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
  2. 2. Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Methods in Molecular Biology Published:2019


Abstract

Various natural and synthetic biomaterials have been applied as skin substitutes for regenerating damaged skin. Here, we describe a straightforward method for fabrication of a tissue-engineered skin substitute by seeding human fetal fibroblasts on acellular human amniotic membrane (HAM). Fetal fibroblasts are achieved from the skin of normal and non-macerated fetus of 11–14 weeks old after spontaneous pregnancy termination. Acellular HAM is obtained by separation of the outer membrane of the chorion and removing its epithelial cells. © Springer Science+Business Media New York 2018.
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