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Decellularized Amniotic Membrane Scaffolds Improve Differentiation of Ipscs to Functional Hepatocyte-Like Cells Publisher Pubmed



Abazari MF1 ; Soleimanifar F2 ; Enderami SE3, 4 ; Nasiri N5 ; Nejati F5 ; Mousavi SA6 ; Soleimani M7 ; Kiani J8 ; Ghoraeian P1 ; Kehtari M4, 9
Authors
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Authors Affiliations
  1. 1. Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
  2. 2. Department of Medical Biotechnology, Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran
  3. 3. Immunogenetics Research Center, Department of Medical Biotechnolmicroogy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  4. 4. Department of Stem Cell Biology, Stem Cell Technology Research Center, Tehran, Iran
  5. 5. Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
  6. 6. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
  7. 7. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  8. 8. Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  9. 9. Department of Developmental Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran

Source: Journal of Cellular Biochemistry Published:2020


Abstract

Human-induced pluripotent stem cells-derived hepatocyte-like cells (hiPSCs-HLCs) holds considerable promise for future clinical personalized therapy of liver disease. However, the low engraftment of these cells in the damaged liver microenvironment is still an obstacle for potential application. In this study, we explored the effectiveness of decellularized amniotic membrane (dAM) matrices for culturing of iPSCs and promoting their differentiation into HLCs. The DNA content assay and histological evaluation indicated that cellular and nuclear residues were efficiently eliminated and the AM extracellular matrix component was maintained during decelluarization. DAM matrices were developed as three-dimensional scaffolds and hiPSCs were seeded into these scaffolds in defined induction media. In dAM scaffolds, hiPSCs-HLCs gradually took a typical shape of hepatocytes (polygonal morphology). HiPSCs-HLCs that were cultured into dAM scaffolds showed a higher level of hepatic markers than those cultured in tissue culture plates (TCPs). Moreover, functional activities in term of albumin and urea synthesis and CYP3A activity were significantly higher in dAM scaffolds than TCPs over the same differentiation period. Thus, based on our results, dAM scaffold might have a considerable potential in liver tissue engineering, because it can improve hepatic differentiation of hiPSCs which exhibited higher level of the hepatic marker and more stable metabolic functions. © 2019 Wiley Periodicals, Inc.
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