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Inactivated Herpes Simplex Virus-1 Vaccine Formulated in Aqueous and Alcoholic Extracts of Propolis Boosts Cellular and Igg Responses Publisher



Mojarab S1, 2 ; Karimi P3 ; Shahbazzadeh D4 ; Moghbeli M2 ; Bagheri KP4 ; Parikhani AB4 ; Dehghan R4 ; Zafari E1 ; Moravej A5 ; Pouriayevali MH6, 7 ; Mirtalebi SF8 ; Pakjoo M1 ; Yazdani S9, 10 ; Abdollahpouralitappeh M11 Show All Authors
Authors
  1. Mojarab S1, 2
  2. Karimi P3
  3. Shahbazzadeh D4
  4. Moghbeli M2
  5. Bagheri KP4
  6. Parikhani AB4
  7. Dehghan R4
  8. Zafari E1
  9. Moravej A5
  10. Pouriayevali MH6, 7
  11. Mirtalebi SF8
  12. Pakjoo M1
  13. Yazdani S9, 10
  14. Abdollahpouralitappeh M11
  15. Mahdavi M1, 12, 13
Show Affiliations
Authors Affiliations
  1. 1. Advanced Therapy Medicinal Product (ATMP) Department, Breast Cancer Research Center, Motamed Cancer Institute, Academic Center for Education, Culture, and Research (ACECR), Tehran, Iran
  2. 2. Department of Biology, Islamic Azad University, Damghan Branch, Damghan, Iran
  3. 3. Department of Biochemistry, Faculty of Basic Sciences, Islamic Azad University, Central Tehran Branch, Tehran, Iran
  4. 4. Venom and Biotherapeutics Molecules Laboratory, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran
  5. 5. Department of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
  6. 6. COVID-19 National Reference Laboratory, Pasteur Institute of Iran, Tehran, Iran
  7. 7. Department of Arboviruses and Viral Hemorrhagic Fevers, National Reference Laboratory, Pasteur Institute of Iran, Tehran, Iran
  8. 8. Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran
  9. 9. Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  10. 10. Department of Microbiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  11. 11. Cellular and Molecular Biology Research Center, Larestan University of Medical Sciences, Larestan, Iran
  12. 12. Recombinant Vaccine Research Center, Tehran University of Medical Sciences, Tehran, Iran
  13. 13. Immunotherapy Group, The Institute of Pharmaceutical Science (TIPS), Tehran University of Medical Science, Tehran, Iran

Source: Iranian Journal of Basic Medical Sciences Published:2024


Abstract

Objective(s): In this study, the adjuvant activity of aqueous and alcoholic extracts of propolis was examined on the inactivated herpes simplex virus-1 (HSV-1). Materials and Methods: BALB/C mice were administered with inactivated (HSV-1; the KOS strain) plus alcoholic and aqueous extracts, followed by assessment of the cellular and humoral immune responses. Results: Alcoholic and aqueous extracts, as an adjuvant, revealed a significant increase in lymphocyte proliferation and cytotoxic T lymphocyte (CTL) responses versus the HSV-1 group. In addition, HSV-1 plus alcoholic extract showed a remarkable increase in IFN-γ cytokine and IFN-γ/IL-4 ratio. On the other hand, both alcoholic and aqueous extracts in the HSV-1 vaccine suppressed the IL-4 cytokine response as compared with the HSV-1 vaccine. In addition, HSV-1 plus alcoholic extract showed a significant increment in IgG1, IgG2a, and IgG2b isotypes as compared with the HSV-1 vaccine. Conclusion: Propolis extracts seem to modulate the immune response against inactivated HSV-1 model and can be used as a suitable vaccine adjuvant or a component of a complex adjuvant against infectious diseases. © 2024 Mashhad University of Medical Sciences. All rights reserved.
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