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Renal Cell Carcinoma Derived Exosome As Natural Nanoparticles Inhibits T Cell Proliferation and Induces Their Apoptosis Publisher



Babakhani D1 ; Daneshdoust D2 ; Baharvand A3 ; Mivefroshan A4 ; Raeisi I5 ; Zuhair Talib Alnaqeeb B6 ; Tavakoli F7
Authors
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Authors Affiliations
  1. 1. Hematology and Oncology, Hematology, Oncology Research Center and Stem Cell Transplantation (HORCST), Dr. Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
  3. 3. Student Research Committee, Arak University of Medical Sciences, Arak, Iran
  4. 4. Nephrology and Renal Transplant Research Center, Urmia University of Medical Science, Urmia, Iran
  5. 5. Department of Anesthesiology, Anesthesiology and Critical Care Research Centre, Emam Hossein Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Anesthesia Technology Department, Al-Turath University College, Al Mansour, Baghdad, Iraq
  7. 7. Nephrology, Tehran University of Medical Sciences, Shariati Hospital, Nephrology ward, Tehran, Iran

Source: Nanomedicine Research Journal Published:2023


Abstract

Objective(s): To evaluate the anti-tumor effect of the renal cell carcinoma ACHN cell-exosome on human T cell biological parameters. Methods: Human T cell was isolated from healthy donors. ACHN-cell derived exosome was procured and expression of the CD9 and CD63 was assessed using western blotting. The effects of the ACHN- exosome on T cell proliferation was measured using MTT assay within 24, 48, 72 and 96 hours of treatment with 500-2000 ng/ml exosome. Also, the apoptotic cell percentages in treated cells were assessed by Annexin/PI staining and flowcytometry upon exposure with 500 and 2000 ng/ml exosome within 96 hours of treatment. Results: According to results, exosome therapy inhibited T cell proliferation more evidently at higher concentration. Also, treatment with exosome caused an improvement in the apoptosis of the T cell within 96 hours of treatment. Conclusions: Concerning the results, ACHN- exosome has negative effect on T cell viability and proliferation, facilitating immune evasion. © 2023 The Author(s).
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