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The Safety Property of P-D-Mannuronic Acid (M2000) As a Novel Immunosuppressive Agent on Differentiation, Maturation and Function of Human Dendritic Cells Publisher Pubmed



Fard NA1 ; Tabrizian N1 ; Mirzaei R2 ; Motamed N3 ; Zavareh FT4 ; Mirshafiey A4
Authors
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Authors Affiliations
  1. 1. Department of Cellular and Molecular Biology, Kish International Campus, University of Tehran, Tehran, Iran
  2. 2. Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Iran
  3. 3. Department of Cell and Molecular Biology, Tehran University, Tehran, Iran
  4. 4. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Current Drug Discovery Technologies Published:2016


Abstract

The study's background and aim: In this investigation, the safety property of M2000 (P-D-mannuronic acid) on differentiation, maturation and function of dendritic cells, was determined. P-D-mannuronic acid, as a novel immunosuppressive and antiinflammatory agent, has been tested in various experimental models. In addition, DC-based immunosuppressive drugs can suppress the progression of autoimmune diseases, although, their notable side effects in increasing the risk of infectious diseases and cancers should be considered. Materials and Methods: The effect of M2000 on differentiation, maturation and function of dendritic cells was examined. To investigate how M2000 affects human dendritic cells (DC) in a defined inflammatory environment, human peripheral blood mononuclear cells (PBMC) were isolated from healthy blood and monocytes were purified using anti-CD14 microbeads. Monocytes were incubated with M2000 in two different doses (6 and 12 ^g/well) along with adding the granulocyte-macrophage colony-stimulating factor (GM- CSF) and interleukin-4 for inducing monocytes to immature DC and lipopolysaccharide for running DC maturation. The differentiation, maturation and function of dendritic cells were examined with flow cytometry and ELISA method. Result: The results demonstrate that M2000 has no significant side on differentiation, maturation and function of dendritic cells in immature DC and mature DC process in vitro. Conclusion: Our findings show that P-D-mannuronic acid (m2000) as a safe agent had no adverse effect on differentiation, maturation and function of dendritic cells which might be recommended as a novel immunosuppressive agent with no or fewer side effects in increasing the risk of infectious diseases and cancers. © 2016 Bentham Science Publishers.
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