Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Efficacy and Safety of G2013 As a Novel Immunosuppressive Agent on Differentiation, Maturation and Function of Human Dendritic Cells



Arjomand Fard N1 ; Tabrizian N1 ; Mirzaei R2 ; Hadjati J2 ; Tofighi Zavareh F3 ; Salehi Nodeh AR3 ; Mirshafiey A3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Dept. of Cellular and Molecular Biology, Kish International Campus, University of Tehran, Tehran, Iran
  2. 2. Dept. of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Dept. of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Public Health Published:2017

Abstract

Background: The expanse of dendritic cells (DC) differentiation plays an important role in determining immune response. DC-based immunosuppressive drugs have notable side effects in increasing the risk of infectious diseases and cancers. G2013, as a novel anti-inflammatory and immunosuppressive agent, has been tested in experimental model of multiple sclerosis. The aim of this study was to conduct the safety property of G2013 on dendritic cells biology. Methods: The effect of G2013 on differentiation, maturation, and function of dendritic cells was examined at Tehran University in 2014. To investigate how G2013 affects human dendritic cells (DC) in a defined inflammatory environment, human peripheral blood mononuclear cells (PBMC) were isolated from healthy blood. Monocytes were then purified using anti-CD14 microbeads. Monocytes were treated with G2013 in two different doses (6 and 12 μg/well) along with adding granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 for inducing monocytes to immature DC and adding lipopolysaccharide for running DC maturation. Differentiation, maturation, and function of dendritic cells were examined with flow cytometry and ELISA. Results: G2013 therapy had no significant effect on CD83, CD86 and DR expression, as well as IL-10 and IL-12 cytokine levels and it, has no remarkable side on differentiation, maturation and function of dendritic cells in immature DC and mature DC process in vitro. Conclusion: G2013 is a safe agent with no adverse effect on differentiation, maturation, and function of dendritic cells. It may be recommended as a novel immunosuppressive agent with no or little side effect in increasing the risk of infectious diseases and cancers. © 2017, Iranian Journal of Public Health. All rights reserved.
Related Docs
View other Related Docs
1. The Safety Property of P-D-Mannuronic Acid (M2000) As a Novel Immunosuppressive Agent on Differentiation, Maturation and Function of Human Dendritic Cells, Current Drug Discovery Technologies (2016)
2. A Review on Defects of Dendritic Cells in Common Variable Immunodeficiency, Endocrine# Metabolic and Immune Disorders - Drug Targets (2017)
3. The Biology of Β-D-Mannuronic Acid (M2000) on Human Dendritic Cell Based on Microrna-155 and Microrna-221, Current Drug Discovery Technologies (2017)
4. The Opposite Effects of Dna and Protein Components of Listeria Monocytogenes and Toxoplasma Gondii on Immunologic Characteristics of Dendritic Cells, Iranian Journal of Allergy# Asthma and Immunology (2015)
5. Evaluation of the Effect of Α-L-Guluronic Acid (G2013) on Cox-1, Cox-2 Activity and Gene Expression for Introducing This Drug As a Novel Nsaid With Immunomodulatory Property, Recent Patents on Inflammation and Allergy Drug Discovery (2018)
6. Preclinical and Pharmacotoxicology Evaluation of Α-L-Guluronic Acid (G2013) As a Non-Steroidal Anti-Inflammatory Drug With Immunomodulatory Property, Immunopharmacology and Immunotoxicology (2017)
7. The Immune System, Clinical Immunology (2022)
8. Immunology of Cutaneous Tumors and Immunotherapy for Melanoma, Cancer Immunology: Cancer Immunotherapy for Organ-Specific Tumors (2015)
Experts (# of related papers)
View all Related Experts
Nima Rezaei (4)
Laleh Sharifi (3)
Other Related Docs
9. Immunomodulatory Effect of G2013 (Α-L-Guluronic Acid) on the Tlr2 and Tlr4 in Human Mononuclear Cells, Current Drug Discovery Technologies (2018)
10. Effects of Guluronic Acid (G2013) on Ship1, Socs1 Induction and Related Molecules in Tlr4 Signaling Pathway, International Immunopharmacology (2018)
11. The Immunomodulatory Role of G2013 (Α-L-Guluronic Acid) on the Expression of Tlr2 and Tlr4 in Ht29 Cell Line, Current Drug Discovery Technologies (2019)
12. Microbial Metabolite Effects on Tlr to Develop Autoimmune Diseases, Toxin Reviews (2019)
13. Herbal Medicine Imod Suppresses Lps-Induced Production of Proinflammatory Cytokines in Human Dendritic Cells, Frontiers in Pharmacology (2015)
14. P435 Her2/Neu-Derived Peptide Conjugated to Liposomes Containing Dope As an Effective Prophylactic Vaccine Formulation for Breast Cancer, Artificial Cells# Nanomedicine and Biotechnology (2019)
15. Inhibitory Effect of G2013 Molecule As a Novel Immunomodulatory Agent, on Mir-155 Gene Expression in Hek-Blue Htlr4 Cell Line, European Journal of Inflammation (2016)
16. Identification of Proteins Derived From Listeria Monocytogenes Inducing Human Dendritic Cell Maturation, Tumor Biology (2016)
17. An in Vitro Evaluation of Anti-Aging Effect of Guluronic Acid (G2013) Based on Enzymatic Oxidative Stress Gene Expression Using Healthy Individuals Pbmcs, Biomedicine and Pharmacotherapy (2017)
18. The Role of Dendritic Cells in Central Nervous System Autoimmunity: Focusing on Multiple Sclerosis and Emerging Therapeutics Targeting Dendritic Cells, Journal of Pediatric Biochemistry (2016)
19. Identification of Toxoplasma Gondii Protein Fractions Induce Immune Response Against Melanoma in Mice, APMIS (2015)