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The Biology of Β-D-Mannuronic Acid (M2000) on Human Dendritic Cell Based on Microrna-155 and Microrna-221 Publisher Pubmed



Tabrizian N1 ; Fard NA1 ; Farazmand A4 ; Eftekhari R2 ; Zavareh FT3 ; Mirshafiey A3
Authors
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Authors Affiliations
  1. 1. Department of Cellular and Molecular Biology, Kish International Campus, University of Tehran, Tehran, Iran
  2. 2. Immunology Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Box: 14155-6446, Tehran, Iran
  4. 4. Department of Cellular and Molecular Biology, School of Biology University, College of Science, Tehran, Iran

Source: Current Drug Discovery Technologies Published:2017


Abstract

Background: The aim of this study was to evaluate the effect of β-D-mannuronic acid (M2000) on related miRNAs to dendritic cells (DCs) differentiation. DC-based immunosuppressive drugs can suppress the progression of autoimmune diseases, however, their notable side effects in increasing the risk of infectious diseases and cancers should be considered. The β-D-mannuronic acid, as a novel non-steroidal anti-inflammatory agent, has been tested in various experimental models. Method: The effect of M2000 on expression of miRNA-155 and miRNA-221 was examined. To investigate how M2000 affects differentiation of human dendritic DCs in a defined inflammatory environment, human peripheral blood mononuclear cells were isolated from healthy blood and the monocytes were purified using anti-CD 14 microbeads. The so isolated monocytes were subsequently incubated in the presence of M2000 in two different doses (3 and 6 mMol/well) adding granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 for inducing monocytes to immature DC and lipopolysaccharide for running DC differentiation. The expression of miRNA-155 and miRNA-221 were examined with Real Time PCR. Results: The results demonstrate that M2000 has no significant side effect on expression of miR-155 and miR-221 in both immature DC and mature DC process in vitro. Conclusion: Our findings show that β-D-mannuronic acid is a safe agent which has no adverse effect on regulatory miRNA-155 and miRNA-221 in dendritic cells. © 2017 Bentham Science Publishers.
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