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Chitosan-Intercalated Montmorillonite/Poly(Vinyl Alcohol) Nanofibers As a Platform to Guide Neuronlike Differentiation of Human Dental Pulp Stem Cells Publisher Pubmed



Ghasemi Hamidabadi H2 ; Rezvani Z4 ; Nazm Bojnordi M3 ; Shirinzadeh H5 ; Seifalian AM6 ; Joghataei MT7 ; Razaghpour M8 ; Alibakhshi A9 ; Yazdanpanah A11 ; Salimi M10 ; Mozafari M4, 7, 12 ; Urbanska AM13 ; Reis RL14 ; Kundu SC14 Show All Authors
Authors
  1. Ghasemi Hamidabadi H2
  2. Rezvani Z4
  3. Nazm Bojnordi M3
  4. Shirinzadeh H5
  5. Seifalian AM6
  6. Joghataei MT7
  7. Razaghpour M8
  8. Alibakhshi A9
  9. Yazdanpanah A11
  10. Salimi M10
  11. Mozafari M4, 7, 12
  12. Urbanska AM13
  13. Reis RL14
  14. Kundu SC14
  15. Gholipourmalekabadi M7, 9, 12
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  2. 2. Immunogenetic Research Center, Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  3. 3. Molecular and Cell Biology Research Center, Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  4. 4. Bioengineering Research Group, Nanotechnology and Advanced Materials Department, Materials and Energy Research Center (MERC), P.O. Box 14155-4777, Tehran, Iran
  5. 5. Semiconductor Department, Materials and Energy Research Center (MERC), P.O. Box 14155-4777, Tehran, Iran
  6. 6. Nanotechnology and Regenerative Medicine Commercialisation Centre (Ltd), London BioScience Innovation Centre, London, NW1 0NH, United Kingdom
  7. 7. Cellular and Molecular Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran
  8. 8. Amirkabir University of Technology, Textile Department, No. 424, Tehran, Iran
  9. 9. Biotechnology Department, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  10. 10. Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  11. 11. Biomaterials Group, Faculty of Biomedical Engineering (Center of Excellence), Amirkabir University of Technology, P.O. Box 15875-4413, Tehran, Iran
  12. 12. Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  13. 13. Division of Digestive and Liver Disease, Department of Medicine, Herbert Irving Comprehensive Cancer Center, Columbia University, New York, 10032, NY, United States
  14. 14. 3Bs Research Group, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, AvePark, Barco, Guimaraes, 4805-017, Portugal

Source: ACS Applied Materials and Interfaces Published:2017


Abstract

In this study, we present a novel chitosan-intercalated montmorillonite/poly(vinyl alcohol) (OMMT/PVA) nanofibrous mesh as a microenvironment for guiding differentiation of human dental pulp stem cells (hDPSCs) toward neuronlike cells. The OMMT was prepared through ion exchange reaction between the montmorillonite (MMT) and chitosan. The PVA solutions containing various concentrations of OMMT were electrospun to form 3D OMMT-PVA nanofibrous meshes. The biomechanical and biological characteristics of the nanofibrous meshes were evaluated by ATR-FTIR, XRD, SEM, MTT, and LDH specific activity, contact angle, and DAPI staining. They were carried out for mechanical properties, overall viability, and toxicity of the cells. The hDPSCs were seeded on the prepared scaffolds and induced with neuronal specific differentiation media at two differentiation stages (2 days at preinduction stage and 6 days at induction stage). The neural differentiation of the cells cultured on the meshes was evaluated by determining the expression of Oct-4, Nestin, NF-M, NF-H, MAP2, and βIII-tubulin in the cells after preinduction, at induction stages by real-time PCR (RT-PCR) and immunostaining. All the synthesized nanofibers exhibited a homogeneous morphology with a favorable mechanical behavior. The population of the cells differentiated into neuronlike cells in all the experimental groups was significantly higher than that in control group. The expression level of the neuronal specific markers in the cells cultured on 5% OMMT/PVA meshes was significantly higher than the other groups. This study demonstrates the feasibility of the OMMT/PVA artificial nerve graft cultured with hDPSCs for regeneration of damaged neural tissues. These fabricated matrices may have a potential in neural tissue engineering applications. © 2017 American Chemical Society.
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