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Role of Cerebrospinal Fluid in Differentiation of Human Dental Pulp Stem Cells Into Neuron-Like Cells Publisher



Goudarzi GG1, 2 ; Hamidabadi HG3 ; Bojnordi MN3 ; Hedayatpour A4 ; Niapour A5 ; Zahiri M6 ; Absalan F7 ; Darabi S8
Authors
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Authors Affiliations
  1. 1. Department of Brain and Cognitive Sciences, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran
  2. 2. Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari
  3. 3. Immunogenetic Research Center, Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari
  4. 4. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran
  5. 5. Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil
  6. 6. Department of Anatomical Sciences, School of Medical Sciences, Bushehr University of Medical Sciences, Bushehr
  7. 7. Abadan School of Medical Sciences, Abadan
  8. 8. Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

Source: Anatomy and Cell Biology Published:2020


Abstract

Human dental pulp stem cells (hDPSCs) could be differentiated into neuron like-cells under particular microenvironments. It has been reported that a wide range of factors, presented in cerebrospinal fluid (CSF), playing part in neuronal differentiation during embryonic stages, we herein introduce a novel culture media complex to differentiate hDPSCs into neuron-like cells. The hDPSCs were initially isolated and characterized. The CSF was prepared from the Cisterna magna of 19-day-old Wistar rat embryos, embryonic cerebrospinal fluid (E-CSF). The hDPSCs were treated by 5% E-CSF for 2 days, then neurospheres were cultured in DMEM/F12 supplemented with 10-6 μm retinoic acid (RA), glialderived neurotrophic factor and brain-derived neurotrophic factor for 6 days. The cells which were cultured in basic culture medium were considered as control group. Morphology of differentiated cells as well as process elongation were examined by an inverted microscope. In addition, the neural differentiation markers (Nestin and MAP2) were studied employing immunocytochemistry. Neuronal-like processes appeared 8 days after treatment. Neural progenitor marker (Nestin) and a mature neural marker (MAP2) were expressed in treated group. Moreover Nissl bodies were found in the cytoplasm of treated group. Taking these together, we have designed a simple protocol for generating neuron-like cells using CSF from the hDPSCs, applicable for cell therapy in several neurodegenerative disorders including Alzheimer’s disease. Copyright © 2020. Anatomy & Cell Biology
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