Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Curcumin-Loaded Phb/Plla Nanofibrous Scaffold Supports Osteogenesis in Adipose-Derived Stem Cells in Vitro Publisher



Abazari MF1 ; Zare Karizi S2 ; Hajatibirgani N3 ; Kohandani M4 ; Torabinejad S5 ; Nejati F6 ; Nasiri N6 ; Maleki MH7 ; Mohajerani H8 ; Mansouri V9
Authors
Show Affiliations
Authors Affiliations
  1. 1. Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Biology, Varamin Pishva Branch, Islamic Azad University, Varamin, Iran
  3. 3. Department of Biology, Faculty of Science and Research, Islamic Azad University, Tehran, Iran
  4. 4. Department of Biology, Faculty of Biological Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran
  5. 5. Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
  6. 6. Institute of Molecular Biology, Vrije Universiteit Brussel, Brussels, Belgium
  7. 7. Department of Biology, School of Sciences, University of Tehran, Tehran, Iran
  8. 8. Department of Oral & Maxillofacial Surgery, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  9. 9. Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Polymers for Advanced Technologies Published:2021


Abstract

One of the most important goals of bone tissue engineering is to regenerate damaged tissue and improve its function by making three-dimensional scaffolds. This role becomes more important and efficient when it can release a biologically active factor at the site of the lesion. In the present study, poly(3-hydroxybutyrate)/poly(l-lactic acid) nanofibers (PHB/PLLA) and curcumin-incorporated PHB/PLLA nanofibers (cur-PHB/PLLA) were fabricated using electrospinning. After morphological and biological characterization, and Curcumin release assessment, the osteogenic differentiation potential of the human adipose–derived stem cells (ADSCs) was investigated with the evaluation of the common osteogenic markers. Fabricated nanofibers were smooth, bead free, and with continuous release of curcumin. The biocompatibility of the fabricated nanofibers was improved while incorporated with curcumin compared to the empty nanofibers. Besides, evaluation of the alkaline phosphatase (ALP) activity, calcium content, and osteogenic-related gene expression revealed that the PHB/PLLA mat can support osteogenic differentiation of the ADSCs more efficiently while incorporated with curcumin. Based on the results, it can be concluded that cur-PHB/PLLA has promising potential for use in the treatment of the bone lesions and/or bone tissue engineering. © 2021 John Wiley & Sons Ltd.
Related Docs
View other Related Docs
1. Poly (Glycerol Sebacate) and Polyhydroxybutyrate Electrospun Nanocomposite Facilitates Osteogenic Differentiation of Mesenchymal Stem Cells, Journal of Drug Delivery Science and Technology (2021)
2. Incorporated-Bfgf Polycaprolactone/Polyvinylidene Fluoride Nanocomposite Scaffold Promotes Human Induced Pluripotent Stem Cells Osteogenic Differentiation, Journal of Cellular Biochemistry (2019)
3. Promoted Osteogenic Differentiation of Human Induced Pluripotent Stem Cells Using Composited Polycaprolactone/Polyvinyl Alcohol/Carbopol Nanofibrous Scaffold, Journal of Drug Delivery Science and Technology (2022)
4. Acceleration of Osteogenic Differentiation by Sustained Release of Bmp2 in Plla/Graphene Oxide Nanofibrous Scaffold, Polymers for Advanced Technologies (2021)
5. Clinical Challenges in Bone Tissue Engineering - a Narrative Review, Bone (2025)
6. Scrophularia Striata Extract Incorporated Nanofibrous Scaffold Improved Osteogenic Differentiation of Mesenchymal Stem Cells, Journal of Drug Delivery Science and Technology (2023)
7. Bone Using Stem Cells for Maxillofacial Bone Disorders: A Systematic Review and Meta-Analysis, Advances in Experimental Medicine and Biology (2023)
8. The Food and Biomedical Applications of Curcumin-Loaded Electrospun Nanofibers: A Comprehensive Review, Critical Reviews in Food Science and Nutrition (2023)
Experts (# of related papers)
View all Related Experts
Reza Masaeli (1)
Nima Rezaei (1)
Other Related Docs
9. Improved Osteogenic Differentiation of Human Induced Pluripotent Stem Cells Cultured on Polyvinylidene Fluoride/Collagen/Platelet-Rich Plasma Composite Nanofibers, Journal of Cellular Physiology (2020)
10. Photoreactive Hydrogels Based on Type I Collagen Extracted From Different Sources As Scaffolds for Tissue Engineering Applications: A Comparative Study, Materialia (2023)
11. Biologically Modified Electrospun Polycaprolactone Nanofibrous Scaffold Promotes Osteogenic Differentiation, Journal of Drug Delivery Science and Technology (2022)
12. Polyethylene Oxide-Chitosan-Doxorubicin/Polycaprolactone-Chitosan-Curcumin Ph-Sensitive Core/Shell Nanofibrous Mats for the Treatment of Breast Cancer: Fabrication, Characterization and in Vitro and in Vivo Evaluation, International Journal of Biological Macromolecules (2025)
13. The Bilayer Skin Substitute Based on Human Adipose-Derived Mesenchymal Stem Cells and Neonate Keratinocytes on the 3D Nanofibrous Pcl-Platelet Gel Scaffold, Polymer Bulletin (2022)
14. Curcumin in Tissue Engineering: A Traditional Remedy for Modern Medicine, BioFactors (2019)
15. Tissue Engineered Skin Substitutes, Advances in Experimental Medicine and Biology (2018)
16. Different Osteogenic Differentiation Potential of Mesenchymal Stem Cells on Three Different Polymeric Substrates, Gene (2020)
17. Micro-Rna-Incorporated Electrospun Nanofibers Improve Osteogenic Differentiation of Human-Induced Pluripotent Stem Cells, Journal of Biomedical Materials Research - Part A (2020)
18. Chitosan-Intercalated Montmorillonite/Poly(Vinyl Alcohol) Nanofibers As a Platform to Guide Neuronlike Differentiation of Human Dental Pulp Stem Cells, ACS Applied Materials and Interfaces (2017)
19. Bone Tissue Engineering: Adult Stem Cells in Combination With Electrospun Nanofibrous Scaffolds, Journal of Cellular Physiology (2018)
20. 3D Printing in Oral & Maxillofacial Surgery, 3D Printing in Oral & Maxillofacial Surgery (2021)
21. The Effect of Curcumin on the Differentiation of Mesenchymal Stem Cells Into Mesodermal Lineage, Molecules (2019)