Association of Polymorphisms in Nucleotide Excision Repair Pathway Genes With Susceptibility to Cutaneous Melanoma; [Vztah Mezi Polymorfizmy Genu Nukleotidove Excizni Reparace a Nachylnosti Ke Koznimu Melanomu] Publisher Pubmed



Hashemzehi A¹ ; Ghadyani M² ; Asadian F³ ; Dastgheib SA⁴ ; Kargar S⁵ ; Neamatzadeh H^{6, 7} ; Akbarian E⁸ ; Emarati A⁸ Show Affiliations
Source: Klinicka Onkologie Published:2021

Abstract

Background: The effects of single nucleotide polymorphisms (SNPs) at nucleotide excision repair (NER) pathway on susceptibility to cutaneous melanoma (CM) are of great interest. To date, several epidemiological studies have evaluated whether the XPC, XPD, XPG and XPF polymor-phisms are associated with CM. However, those studies results are controversial or inconclusive. Therefore, we conducted a study to evaluate the association of seven frequently investigated NER pathway polymorphisms with CM risk. Methods: A total of 150 patients diagnosed with CM and 150 healthy controls were enrolled in the study. Seven SNPs in the NER pathway including XPC (Lys939Gln and Ala499Val), XPD (Lys157Gln, Asp272Asn, and Arg751Arg), XPG (Asp1104His) and XPF (Arg415Gln) were analyzed by polymerase chain reaction restriction fragment length polymorphism assay. Results: There was no a significant association between XPC Lys939Gln, Ala499Val; XPD Asp272Asn, Arg751Arg, Arg751Arg; XPF Arg415Gln; and XPG Asp1104His poly-morphisms and an increased risk of CM. Conclusions: This study results revealed that the XPC, XPD, XPG and XPF polymorphisms were not risk factor for susceptibility to CM. However, more well-designed with larger sample size studies in different populations are necessary to further evaluate and validate our results. Future studies which take into account gene-gene and gene--environment interactions are warranted for more precise evidence and further elucidation of the underlying mechanism of CM. © 2021, Czech Medical Association J.E. Purkyne. All rights reserved.