Graphite Nanocrystals Coated Paper-Based Electrode for Detection of Sars-Cov-2 Gene Using Dna-Functionalized Au@Carbon Dot Core

Graphite Nanocrystals Coated Paper-Based Electrode for Detection of Sars-Cov-2 Gene Using Dna-Functionalized Au@Carbon Dot Core–Shell Nanoparticles Publisher

Ali Farzin M¹ ; Abdoos H¹ ; Saber R²

Show Affiliations

1. Department of Nanotechnology, Faculty of New Sciences and Technologies, Semnan University, P.O. Box: 35131-19111, Semnan, Iran
2. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Microchemical Journal Published:2022

Abstract

Currently, the development of biosensors is an urgent need due to the rapid spread of SARS-CoV-2 and the limitations of current standard methods for the diagnosis of COVID-19. Hence, many researchers have focused on the design of high-performance biosensors for measuring coronavirus genes. In this study, a voltammetric genosensor was developed for the determination of SARS-CoV-2 RdRP gene based on the format of cDNA probe/Au@CD core–shell NPs/graphite nanocrystals (GNCs)/paper electrode. For the first time, graphite nanocrystals were used in the electrochemical biosensor design. This genosensor was exposed to different concentrations of virus gene and then the hybridization between cDNA probe and RdRP gene was monitored by redox-active toluidine blue (TB). With increasing the RdRP concentration, the reduction peak current of TB enhanced in a linear range of 0.50 pM-12.00 nM according to the regression equation of I (μA) = 7.60 log CRdRP (pM) + 25.78. The repeatability with a RSD of 2.2% clearly exhibited that the response of modified electrode is stable because of the high adhesion of GNC layer on the paper substrate and the high stability of cDNA-Au@CD bioconjugates. The spike-and-recovery studies showed the acceptable recoveries for the sputum samples (>95%). © 2022 Elsevier B.V.

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Reza Saber (3)

Saeid Sarkar (1)

Style	Citing Format
MLA	Ali Farzin M, Abdoos H, Saber R. "Graphite Nanocrystals Coated Paper-Based Electrode for Detection of Sars-Cov-2 Gene Using Dna-Functionalized Au@Carbon Dot Core–Shell Nanoparticles." Microchemical Journal, vol. 179, no. , 2022, pp. -.
APA	Ali Farzin M, Abdoos H, Saber R (2022). Graphite Nanocrystals Coated Paper-Based Electrode for Detection of Sars-Cov-2 Gene Using Dna-Functionalized Au@Carbon Dot Core–Shell Nanoparticles. Microchemical Journal, 179(), -.
Chicago	Ali Farzin M, Abdoos H, Saber R. "Graphite Nanocrystals Coated Paper-Based Electrode for Detection of Sars-Cov-2 Gene Using Dna-Functionalized Au@Carbon Dot Core–Shell Nanoparticles." Microchemical Journal 179, no. (2022): -.
Harvard	Ali Farzin M, Abdoos H, Saber R (2022) 'Graphite Nanocrystals Coated Paper-Based Electrode for Detection of Sars-Cov-2 Gene Using Dna-Functionalized Au@Carbon Dot Core–Shell Nanoparticles', Microchemical Journal, 179(), pp. -.
Vancouver	Ali Farzin M, Abdoos H, Saber R. Graphite Nanocrystals Coated Paper-Based Electrode for Detection of Sars-Cov-2 Gene Using Dna-Functionalized Au@Carbon Dot Core–Shell Nanoparticles. Microchemical Journal. 2022;179():-.
BibTex	@article{ author = {Ali Farzin M and Abdoos H and Saber R}, title = {Graphite Nanocrystals Coated Paper-Based Electrode for Detection of Sars-Cov-2 Gene Using Dna-Functionalized Au@Carbon Dot Core–Shell Nanoparticles}, journal = {Microchemical Journal}, volume = {179}, number = {}, pages = {-}, year = {2022} }
RIS	TY - JOUR AU - Ali Farzin M AU - Abdoos H AU - Saber R TI - Graphite Nanocrystals Coated Paper-Based Electrode for Detection of Sars-Cov-2 Gene Using Dna-Functionalized Au@Carbon Dot Core–Shell Nanoparticles JO - Microchemical Journal VL - 179 IS - SP - EP - PY - 2022 ER -

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