Bioactive Hybrid Metal-Organic Framework (Mof)-Based Nanosensors for Optical Detection of Recombinant Sars-Cov-2 Spike Antigen Publisher Pubmed



Rabiee N^{1, 2} ; Fatahi Y^{3, 4} ; Ahmadi S^{5, 6} ; Abbariki N⁷ ; Ojaghi A⁷ ; Rabiee M⁸ ; Radmanesh F⁹ ; Dinarvand R^{3, 4} ; Bagherzadeh M⁷ ; Mostafavi E^{10, 11} ; Ashrafizadeh M^{12, 13} ; Makvandi P¹⁴ ; Lima EC¹⁵ ; Saeb MR¹⁶ Show Affiliations
Source: Science of the Total Environment Published:2022

Abstract

Fast, efficient, and accurate detection of SARS-CoV-2 spike antigen is pivotal to control the spread and reduce the mortality of COVID-19. Nevertheless, the sensitivity of available nanobiosensors to detect recombinant SARS-CoV-2 spike antigen seems insufficient. As a proof-of-concept, MOF-5/CoNi2S4 is developed as a low-cost, safe, and bioactive hybrid nanostructure via the one-pot high-gravity protocol. Then, the porphyrin, H2TMP, was attached to the surface of the synthesized nanomaterial to increase the porosity for efficient detection of recombinant SARS-CoV-2 spike antigen. AFM results approved roughness in different ranges, including 0.54 to 0.74 μm and 0.78 to ≈0.80 μm, showing good physical interactions with the recombinant SARS-CoV-2 spike antigen. MTT assay was performed and compared to the conventional synthesis methods, including hydrothermal, solvothermal, and microwave-assisted methods. The synthesized nanodevices demonstrated above 88% relative cell viability after 24 h and even 48 h of treatment. Besides, the ability of the synthesized nanomaterials to detect the recombinant SARS-CoV-2 spike antigen was investigated, with a detection limit of 5 nM. The in-situ synthesized nanoplatforms exhibited low cytotoxicity, high biocompatibility, and appropriate tunability. The fabricated nanosystems seem promising for future surveys as potential platforms to be integrated into biosensors. © 2022 Elsevier B.V.