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Evaluating the Mir-302B and Mir-145 Expression in Formalin-Fixed Paraffin-Embedded Samples of Esophageal Squamous Cell Carcinoma Pubmed



Tabrizi M1 ; Khalili M2 ; Vasei M3 ; Nouraei N1 ; Mansour Samaei N4 ; Khavanin A5 ; Khajehei M6 ; Mowla SJ1
Authors
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Authors Affiliations
  1. 1. Department of Molecular Genetics, Tarbiat Modares University, Tehran, Iran
  2. 2. Department of Medical Genetics and Molecular Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
  3. 3. Pathology laboratory, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Human Genetics Department, Golestan University of Medical Sciences, Gorgan, Iran
  5. 5. Emergency Medicine Department, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  6. 6. Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran

Source: Archives of Iranian Medicine Published:2015


Abstract

Background: MicroRNAs are involved in key cellular processes regulating, and their misregulation is linked to cancer. The miR-302-367 cluster is exclusively expressed in embryonic stem and carcinoma cells. This cluster also promotes cell reprogramming and stemness process. In contrast, miR-145 is mostly regarded as a tumor suppressor, where it regulates cellular functions such as cell division, differentiation, and apoptosis. By suppressing the main pluripotency factors (OCT4, SOX2, MYC and KLF4), miR-145 silences the self-renewal program in ESCs. Therefore, the main aim of this study is to find a potential link between the expression level of hsa-miR-302b and hsa-miR-145 with tumor vs. non-tumor as well as high-grade vs. low-grade states of the esophageal tissue samples. Methods: A total number of 40 formalin-fixed, paraffin-embedded (FFPE) samples of esophageal squamous-cell carcinoma (ESCC) were obtained, and the tumor and marginal non-tumor areas delineated and punched off by an expert pathologist. Total RNA was extracted with Trizol, and cDNA synthesized using the miRCURY LNA™ Universal RT microRNA PCR Kit. Real-time reverse transcription polymerase chain reaction (RT-PCR) assays were performed using specific LNA-primers and SYBR Green master mix. Results: The expression level of miR-302b failed to show any significant difference, neither between tumor and their non-tumor counterparts, nor among tumors with different grades of malignancies (P > 0.05). In contrast, miR-145 was significantly down regulated in all grades of tumor samples (P < 0.001). However, its expression level could not discriminate between different grades of malignancy (P > 0.05). CONCLUSION: Our data revealed a significant down-regulation of miR-145 in ESCC tissue samples. Based on our ROC curve analysis data (AUC = 0.74, P < 0.001) miR-145 could be regarded as a potential tumor marker for diagnosis of esophageal cancer. © 2015, Academy of Medical Sciences of I.R. Iran. All rights reserved.
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