Constructing a Novel Hypoxia-Inducible Bidirectional Shrna Expression Vector for Simultaneous Gene Silencing in Colorectal Cancer Gene Therapy

Constructing a Novel Hypoxia-Inducible Bidirectional Shrna Expression Vector for Simultaneous Gene Silencing in Colorectal Cancer Gene Therapy Publisher Pubmed

Javan B^{1, 2} ; Shahbazi M^{1, 2, 3}

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1. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Shast Kola road, Gorgan, 4934174516, Iran
2. Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran
3. Arya Tina Gene (ATG), Biopharmaceutical Company, Gorgan, Iran

Source: Cancer Biotherapy and Radiopharmaceuticals Published:2018

Abstract

Background: Nonspecific siRNA expression limits its application in cancer gene therapy. Therefore, a tightly regulated and reversibly inducible RNAi system is required to conditionally control the gene expression. This investigation aims at constructing a hypoxia/colorectal tumor dual-specific bidirectional short hairpin RNA (shRNA) expression vector. Materials and Methods: First, carcinoma embryonic antigen (CEA) promoter designed in two directions. Then, pRNA-bipHRE-CEA vector was constructed by insertion of the vascular endothelial growth factor enhancer between two promoters for hypoxic cancer-specific gene expression. To confirm the therapeutic effect of the dual-specific vector, two shRNA oligonucleotides were inserted in the downstream of each promoter. QRT-polymerase chain reaction and western blot assays were performed to estimate the mRNA and protein expression levels. Results: Both mRNA and protein levels were significantly reduced (50%-60%) in the hypoxic colorectal cancer-treated cells when compared with the controls. Conclusion: The novel bidirectional hypoxia-inducible shRNA expression vector may be efficient in colorectal cancer-specific gene therapy. © 2018, Mary Ann Liebert, Inc.

2. Potential Sirna Molecules for Nucleoprotein and M2/L Overlapping Region of Respiratory Syncytial Virus: In Silico Design, Jundishapur Journal of Microbiology (2016)

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Fatemeh Atyabi (1)

Vahid Salimi (1)

Style	Citing Format
MLA	Javan B, Shahbazi M. "Constructing a Novel Hypoxia-Inducible Bidirectional Shrna Expression Vector for Simultaneous Gene Silencing in Colorectal Cancer Gene Therapy." Cancer Biotherapy and Radiopharmaceuticals, vol. 33, no. 3, 2018, pp. 118-123.
APA	Javan B, Shahbazi M (2018). Constructing a Novel Hypoxia-Inducible Bidirectional Shrna Expression Vector for Simultaneous Gene Silencing in Colorectal Cancer Gene Therapy. Cancer Biotherapy and Radiopharmaceuticals, 33(3), 118-123.
Chicago	Javan B, Shahbazi M. "Constructing a Novel Hypoxia-Inducible Bidirectional Shrna Expression Vector for Simultaneous Gene Silencing in Colorectal Cancer Gene Therapy." Cancer Biotherapy and Radiopharmaceuticals 33, no. 3 (2018): 118-123.
Harvard	Javan B, Shahbazi M (2018) 'Constructing a Novel Hypoxia-Inducible Bidirectional Shrna Expression Vector for Simultaneous Gene Silencing in Colorectal Cancer Gene Therapy', Cancer Biotherapy and Radiopharmaceuticals, 33(3), pp. 118-123.
Vancouver	Javan B, Shahbazi M. Constructing a Novel Hypoxia-Inducible Bidirectional Shrna Expression Vector for Simultaneous Gene Silencing in Colorectal Cancer Gene Therapy. Cancer Biotherapy and Radiopharmaceuticals. 2018;33(3):118-123.
BibTex	@article{ author = {Javan B and Shahbazi M}, title = {Constructing a Novel Hypoxia-Inducible Bidirectional Shrna Expression Vector for Simultaneous Gene Silencing in Colorectal Cancer Gene Therapy}, journal = {Cancer Biotherapy and Radiopharmaceuticals}, volume = {33}, number = {3}, pages = {118-123}, year = {2018} }
RIS	TY - JOUR AU - Javan B AU - Shahbazi M TI - Constructing a Novel Hypoxia-Inducible Bidirectional Shrna Expression Vector for Simultaneous Gene Silencing in Colorectal Cancer Gene Therapy JO - Cancer Biotherapy and Radiopharmaceuticals VL - 33 IS - 3 SP - 118 EP - 123 PY - 2018 ER -

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