Mirna-Mediated Khsrp Silencing Rewires Distinct Post-Transcriptional Programs During Tgf-Β-Induced Epithelial-To-Mesenchymal Transition

Mirna-Mediated Khsrp Silencing Rewires Distinct Post-Transcriptional Programs During Tgf-Β-Induced Epithelial-To-Mesenchymal Transition Publisher Pubmed

Puppo M^{1, 2} ; Bucci G³ ; Rossi M^{1, 2} ; Giovarelli M¹ ; Bordo D¹ ; Moshiri A^{1, 6} ; Gorlero F^{4, 5} ; Gherzi R¹ ; Briata P¹

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1. Gene Expression Regulation Laboratory, IRCCS AOU San Martino-IST, Genova, 16132, Italy
2. DIMES Sezione Biochimica-Universita di Genova, Genova, 16132, Italy
3. Center for Translational Genomics and Bioinformatics, San Raffaele Scientific Institute, Milano, 20132, Italy
4. S.C. Ginecologia e Ostetricia Galliera Hospital, 16128, Genova, Italy
5. School of Medicine, DINOGMI, University of Genova, Genova, 16128, Italy
6. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, 198396-3113, Iran

Source: Cell Reports Published:2016

Abstract

Epithelial-to-mesenchymal transition (EMT) confers several traits to cancer cells that are required for malignant progression. Here, we report that miR-27b-3p-mediated silencing of the single-strand RNA binding protein KHSRP is required for transforming growth factor β (TGF-β)-induced EMT in mammary gland cells. Sustained KHSRP expression limits TGF-β-dependent induction of EMT factors and cell migration, whereas its knockdown in untreated cells mimics TGF-β-induced EMT. Genome-wide sequencing analyses revealed that KHSRP controls (1) levels of mature miR-192-5p, a microRNA that targets a group of EMT factors, and (2) alternative splicing of a cohort of pre-mRNAs related to cell adhesion and motility including Cd44 and Fgfr2. KHSRP belongs to a ribonucleoprotein complex that includes hnRNPA1, and the two proteins cooperate in promoting epithelial-type exon usage of select pre-mRNAs. Thus, TGF-β-induced KHSRP silencing is central in a pathway leading to gene-expression changes that contribute to the cellular changes linked to EMT. © 2016 The Author(s)

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Style	Citing Format
MLA	Puppo M, et al.. "Mirna-Mediated Khsrp Silencing Rewires Distinct Post-Transcriptional Programs During Tgf-Β-Induced Epithelial-To-Mesenchymal Transition." Cell Reports, vol. 16, no. 4, 2016, pp. 967-978.
APA	Puppo M, Bucci G, Rossi M, Giovarelli M, Bordo D, Moshiri A, Gorlero F, Gherzi R, Briata P (2016). Mirna-Mediated Khsrp Silencing Rewires Distinct Post-Transcriptional Programs During Tgf-Β-Induced Epithelial-To-Mesenchymal Transition. Cell Reports, 16(4), 967-978.
Chicago	Puppo M, Bucci G, Rossi M, Giovarelli M, Bordo D, Moshiri A, Gorlero F, Gherzi R, Briata P. "Mirna-Mediated Khsrp Silencing Rewires Distinct Post-Transcriptional Programs During Tgf-Β-Induced Epithelial-To-Mesenchymal Transition." Cell Reports 16, no. 4 (2016): 967-978.
Harvard	Puppo M et al. (2016) 'Mirna-Mediated Khsrp Silencing Rewires Distinct Post-Transcriptional Programs During Tgf-Β-Induced Epithelial-To-Mesenchymal Transition', Cell Reports, 16(4), pp. 967-978.
Vancouver	Puppo M, Bucci G, Rossi M, Giovarelli M, Bordo D, Moshiri A, et al.. Mirna-Mediated Khsrp Silencing Rewires Distinct Post-Transcriptional Programs During Tgf-Β-Induced Epithelial-To-Mesenchymal Transition. Cell Reports. 2016;16(4):967-978.
BibTex	@article{ author = {Puppo M and Bucci G and Rossi M and Giovarelli M and Bordo D and Moshiri A and Gorlero F and Gherzi R and Briata P}, title = {Mirna-Mediated Khsrp Silencing Rewires Distinct Post-Transcriptional Programs During Tgf-Β-Induced Epithelial-To-Mesenchymal Transition}, journal = {Cell Reports}, volume = {16}, number = {4}, pages = {967-978}, year = {2016} }
RIS	TY - JOUR AU - Puppo M AU - Bucci G AU - Rossi M AU - Giovarelli M AU - Bordo D AU - Moshiri A AU - Gorlero F AU - Gherzi R AU - Briata P TI - Mirna-Mediated Khsrp Silencing Rewires Distinct Post-Transcriptional Programs During Tgf-Β-Induced Epithelial-To-Mesenchymal Transition JO - Cell Reports VL - 16 IS - 4 SP - 967 EP - 978 PY - 2016 ER -

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