Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
A Truncated Snail1 Transcription Factor Alters the Expression of Essential Emt Markers and Suppresses Tumor Cell Migration in a Human Lung Cancer Cell Line Publisher Pubmed



Gholami MD1, 2 ; Falak R1, 2 ; Heidari S2 ; Khoshmirsafa M2 ; Kazemi MH2, 3 ; Zarnani AH4, 5 ; Safari E1, 2 ; Tajik N1, 2 ; Kardar GA6, 7
Authors
Show Affiliations
Authors Affiliations
  1. 1. Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Hematopoietic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
  6. 6. Immunology Asthma & Allergy Research Institute, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Recent Patents on Anti-Cancer Drug Discovery Published:2019


Abstract

Background: Epithelial-to-Mesenchymal Transition (EMT) is necessary for metastasis. Zinc-finger domain-containing transcription factors, especially Snail1, bind to E-box motifs and play a crucial role in the induction and regulation of EMT. Objective: We hypothesized if C-terminal region of Snail1 (CSnail1) may competitively bind to E-box and block cancer metastasis. Methods: The CSnail1 gene coding sequence was inserted into the pIRES2-EGFP vector. Following transfection of A549 cells with the designed construct, EMT was induced with TGF-β1 and the expression of essential EMT markers was evaluated by real-time PCR and immunoblotting. We also monitored cell migration. Results: CSnail1 inhibited TGF-β1-induced N-cadherin and vimentin mRNA expression and increased β-catenin expression in transfected TGF-β1-treated A549 cells. A similar finding was obtained in western blotting. CSnail1 also blocked the migration of transfected cells in the scratch test. Conclusion: Transfection of A549 cells with CSnail1 alters the expression of essential EMT markers and consequently suppresses tumor cell migration. These findings confirm the capability of CSnail1 in EMT blocking and in parallel to current patents could be applied as a novel strategy in the prevention of metastasis. © 2019 Bentham Science Publishers.
Related Docs
View other Related Docs
1. Comparison of Snail1, Zeb1, E-Cadherin Expression Levels in Hpv-Induced Cervical Cancer, Iranian Journal of Public Health (2020)
2. The Role of Autophagy in Cancer: From Molecular Mechanism to Therapeutic Window, Frontiers in Immunology (2025)
3. Tlr4 Blockade Using Tak-242 Suppresses Ovarian and Breast Cancer Cells Invasion Through the Inhibition of Extracellular Matrix Degradation and Epithelial-Mesenchymal Transition, European Journal of Pharmacology (2019)
4. Tumor-Associated Macrophages and Epithelial–Mesenchymal Transition in Cancer: Nanotechnology Comes Into View, Journal of Cellular Physiology (2018)
5. Competitive Effect of Overexpressed C-Terminal of Snail-1 (Csnail) in Control of the Growth and Metastasis of Melanoma Cells, Recent Patents on Anti-Cancer Drug Discovery (2024)
6. Epithelial-To-Mesenchymal Transition in Thyroid Cancer: A Comprehensive Review, Endocrine (2019)
7. The Molecular Signature of Breast Cancer Metastasis to Bone, Anti-Cancer Drugs (2016)
8. Harnessing Function of Emt in Cancer Drug Resistance: A Metastasis Regulator Determines Chemotherapy Response, Cancer and Metastasis Reviews (2024)
Experts (# of related papers)
View all Related Experts
Gholamali Kardar (3)
Seyed Hamidollah Ghaffari (3)
Other Related Docs
9. Functions of the Snai Family in Chondrocyte-To-Osteocyte Development, Annals of the New York Academy of Sciences (2021)
10. Lung Cancer and Mirnas: A Possible Remedy for Anti-Metastatic, Therapeutic and Diagnostic Applications, Expert Review of Respiratory Medicine (2017)
11. Transcriptional Regulation of E-Cadherin and Oncoprotein E7 by Valproic Acid in Hpv Positive Cell Lines, Iranian Journal of Basic Medical Sciences (2016)
12. Naturally Occurring Compounds Acting As Potent Anti-Metastatic Agents and Their Suppressing Effects on Hedgehog and Wnt/Β-Catenin Signalling Pathways, Cell Proliferation (2017)
13. Cytoplasmic Expression of Twist1, an Emt-Related Transcription Factor, Is Associated With Higher Grades Renal Cell Carcinomas and Worse Progression-Free Survival in Clear Cell Renal Cell Carcinoma, Clinical and Experimental Medicine (2018)
14. Cobalt Chloride-Induced Hypoxia Can Lead Skbr3 and Hek293t Cell Lines Toward Epithelial-Mesenchymal Transition, Iranian Journal of Allergy# Asthma and Immunology (2022)
15. Deferoxamine Preconditioning to Restore Impaired Hif-1Α-Mediated Angiogenic Mechanisms in Adipose-Derived Stem Cells From Stz-Induced Type 1 Diabetic Rats, Cell Proliferation (2015)
16. Circulating Tumor Cells and Dnas in Prostate Tumors, Liquid Biopsy in Urogenital Cancers and its Clinical Utility (2022)
17. Circulating Levels of the Mirnas, Mir-194, and Mir-29B, As Clinically Useful Biomarkers for Colorectal Cancer, Tumor Biology (2016)
18. Investigation of the Correlation Between Androgen Receptor and Zeb1 and Its Value in Progression of Gastric Cancer, Avicenna Journal of Medical Biotechnology (2020)
19. Mir-216B-5P Inhibits Cell Proliferation in Human Breast Cancer by Down-Regulating Hdac8 Expression, Life Sciences (2019)
20. Invasion and Migration of Mda-Mb-231 Cells Are Inhibited by Block of Ahr and Nfat: Role of Ahr/Nfat1/Β4 Integrin Signaling, Journal of Applied Toxicology (2019)
21. Suppressing Effects of Green Tea Extract and Epigallocatechin-3-Gallate (Egcg) on Tgf-Β- Induced Epithelial-To-Mesenchymal Transition Via Ros/Smad Signaling in Human Cervical Cancer Cells, Gene (2021)
22. Lung Cancer-Associated Brain Metastasis: Molecular Mechanisms and Therapeutic Options, Cellular Oncology (2017)
23. The Role of Epithelial-Mesenchymal Transition Markers in the Prediction of Lymph Node Metastases in Colorectal Cancer, Biomarkers in Medicine (2020)
24. Polymeric Micelles of Peg-Pla Copolymer As a Carrier for Salinomycin Against Gemcitabine-Resistant Pancreatic Cancer, Pharmaceutical Research (2015)
25. Liquid Biopsy in Thyroid Cancer: New Insight, International Journal of Hematology-Oncology and Stem Cell Research (2018)
26. Pathogenesis of Breast Cancer Metastasis to Brain: A Comprehensive Approach to the Signaling Network, Molecular Neurobiology (2016)
27. Mirna-Mediated Khsrp Silencing Rewires Distinct Post-Transcriptional Programs During Tgf-Β-Induced Epithelial-To-Mesenchymal Transition, Cell Reports (2016)
28. Epigenetic of Retinoic Acid Receptor ß2 Gene in Breast Cancer, Epigenetics Territory and Cancer (2015)
29. Overexpression and Translocation of Dynamin 2 Promotes Tumor Aggressiveness in Breast Carcinomas, EXCLI Journal (2020)
30. The Impact of Epithelial-Mesenchymal Transition (Emt) Induced by Metabolic Processes and Intracellular Signaling Pathways on Chemo-Resistance, Metastasis, and Recurrence in Solid Tumors, Cell Communication and Signaling (2024)
31. Enrichment of Cancer Stem-Like Cells by the Induction of Epithelial-Mesenchymal Transition Using Lentiviral Vector Carrying E-Cadherin Shrna in Ht29 Cell Line, Journal of Cellular Physiology (2019)
32. Maml1 and Twist1 Co-Overexpression Promote Invasion of Head and Neck Squamous Cell Carcinoma, Asia-Pacific Journal of Clinical Oncology (2018)
33. Unraveling the Function of Epithelial-Mesenchymal Transition (Emt) in Colorectal Cancer: Metastasis, Therapy Response, and Revisiting Molecular Pathways, Biomedicine and Pharmacotherapy (2023)
34. The Urtica Dioica Extract Enhances Sensitivity of Paclitaxel Drug to Mda-Mb-468 Breast Cancer Cells, Biomedicine and Pharmacotherapy (2016)
35. Mir-483-3P Suppresses the Proliferation and Progression of Human Triple Negative Breast Cancer Cells by Targeting the Hdac8>Oncogene, Journal of Cellular Physiology (2020)
36. The Evaluation of the N-Cadherin Promoter’S Ability to Block Emt by Specific Expression of Diphtheria Toxin in Emt-Induced A549 Cell Lines, Iranian Journal of Allergy# Asthma and Immunology (2024)