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Development of a T Cell-Targeted Sirna Delivery System Against Hiv-1 Using Modified Superparamagnetic Iron Oxide Nanoparticles: An in Vitro Study Publisher Pubmed



Kamalzare S1 ; Iranpur Mobarakeh V2 ; Mirzazadeh Tekie FS3 ; Hajiramezanali M4 ; Riazirad F5 ; Yoosefi S6 ; Normohammadi Z7 ; Irani S7 ; Tavakoli M3 ; Rahimi P2 ; Atyabi F1, 3
Authors
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Authors Affiliations
  1. 1. Department of Pharmaceutics, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
  3. 3. Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
  6. 6. Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Source: Journal of Pharmaceutical Sciences Published:2022


Abstract

In spite of the promising properties of small interfering RNAs (siRNAs) in the treatment of infectious diseases, safe and efficient siRNA delivery to target cells is still a challenge. In this research, an effective siRNA delivery approach (against HIV-1) has been reported using targeted modified superparamagnetic iron oxide nanoparticles (SPIONs). Trimethyl chitosan-coated SPION (TMC-SPION) containing siRNA was synthesized and chemically conjugated to a CD4-specific monoclonal antibody (as a targeting moiety). The prepared nanoparticles exhibited a high siRNA loading efficiency with a diameter of about 85 nm and a zeta potential of +28 mV. The results of the cell viability assay revealed the low cytotoxicity of the optimized nanoparticles. The cellular delivery of the targeted nanoparticles (into T cells) and the gene silencing efficiency of the nanoparticles (containing anti-nef siRNA) were dramatically improved compared to those of nontargeted nanoparticles. In conclusion, this study offers a promising targeted delivery platform to induce gene silencing in target cells. Our approach may find potential use in the design of effective vehicles for many therapeutic applications, particularly for HIV treatment. © 2021 American Pharmacists Association
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