Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Safety of Grafting Acellular Human Corneal Lenticule Seeded With Wharton's Jelly-Derived Mesenchymal Stem Cells in an Experimental Animal Model Publisher Pubmed



Aghamollaei H1, 2 ; Hashemian H3 ; Safabakhsh H2 ; Halabian R4 ; Baghersad M1 ; Jadidi K1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
  2. 2. Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
  3. 3. Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

Source: Experimental Eye Research Published:2021


Abstract

The present study was conducted to evaluate safety of grafting acellular human corneal lenticule seeded with Wharton's Jelly-derived Mesenchymal Stem Cells (WJSC) in an experimental animal model. Human corneal lenticules were decellularized with a rate of about 97% with an acceptable lack of cytotoxicity and relatively intact ultrastructure of the lenticules. 12 rabbits underwent unilateral stromal pocketing with implantation of decellularized lenticules. Implantation was performed for 6 rabbits along with graft recellularization with WJSCs. Rabbits were euthanized after 1 month (n = 6) and 3 months (n = 6) to evaluate progression of graft bio-integration. No clinical rejection sign was detected during the study. Histopathological analysis showed that, grafts were integrated well with the least distortion of surrounding collagen bundles. After 3 months, labeled WJCS was detected representing viability of stem cells in the host. Increased expression of keratocyte-specific markers showed the potential of recruiting WJSCs as keratocyte progenitor cells to reinforce corneal ultrastructure. © 2021 Elsevier Ltd
Related Docs
1. Applications of Mesenchymal Stem Cells in Ocular Surface Diseases: Sources and Routes of Delivery, Expert Opinion on Biological Therapy (2023)
2. Concise Review: Bioengineering of Limbal Stem Cell Niche, Bioengineering (2023)
3. Cell-Based Therapies for Limbal Stem Cell Deficiency: A Literature Review, Annals of Eye Science (2023)
4. Biological Materials Introduced to the Market for Blurred Cornea Regeneration, Regenerative Engineering and Translational Medicine (2023)
5. Intrastromal Versus Subconjunctival Injection of Mesenchymal Stem/Stromal Cells for Promoting Corneal Repair, Ocular Surface (2023)
Experts (# of related papers)
Mohammad Soleimani (4)
Ahmad Masoumi (1)