Safety of Grafting Acellular Human Corneal Lenticule Seeded With Wharton's Jelly-Derived Mesenchymal Stem Cells in an Experimental Animal Model Publisher Pubmed



Aghamollaei H^{1, 2} ; Hashemian H³ ; Safabakhsh H² ; Halabian R⁴ ; Baghersad M¹ ; Jadidi K¹
Source: Experimental Eye Research Published:2021

Abstract

The present study was conducted to evaluate safety of grafting acellular human corneal lenticule seeded with Wharton's Jelly-derived Mesenchymal Stem Cells (WJSC) in an experimental animal model. Human corneal lenticules were decellularized with a rate of about 97% with an acceptable lack of cytotoxicity and relatively intact ultrastructure of the lenticules. 12 rabbits underwent unilateral stromal pocketing with implantation of decellularized lenticules. Implantation was performed for 6 rabbits along with graft recellularization with WJSCs. Rabbits were euthanized after 1 month (n = 6) and 3 months (n = 6) to evaluate progression of graft bio-integration. No clinical rejection sign was detected during the study. Histopathological analysis showed that, grafts were integrated well with the least distortion of surrounding collagen bundles. After 3 months, labeled WJCS was detected representing viability of stem cells in the host. Increased expression of keratocyte-specific markers showed the potential of recruiting WJSCs as keratocyte progenitor cells to reinforce corneal ultrastructure. © 2021 Elsevier Ltd