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Role of Iron Oxide Core of Polymeric Nanoparticles in the Thermosensitivity of Colon Cancer Cell Line Ht-29 Publisher Pubmed



Esmaelbeygi E1 ; Khoei S2, 3 ; Khoee S4 ; Eynali S1
Authors
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Authors Affiliations
  1. 1. Medical Physics and Biomedical Engineering Department, School of Medicine, Tehran University of Medical Sciences (TUMS), Tehran, Iran
  2. 2. Medical Physics Department, School of Medicine, Iran University of Medical Sciences, PO Box 14155-5983, Tehran, Iran
  3. 3. Department of Biophysics, Razi Drug Research Centre, School of Medicine, Iran University of Medical Sciences, PO Box 14155-5983, Tehran, Iran
  4. 4. Polymer Chemistry Department, School of Sciences, University of Tehran, Tehran, Iran

Source: International Journal of Hyperthermia Published:2015


Abstract

Purpose: In this study the effect of PLGA polymeric nanoparticles as a 5-fluorouracil (5-FU) carrier with and without iron oxide core and hyperthermia were investigated on the level of DNA damage in a spheroid culture model of HT-29 colon cancer cell lines by alkaline comet assay.Materials and methods: First, HT-29 colon cancer cells were cultured in vitro as spheroids with a mean diameter of 100 μm. The spheroids were then treated with different concentrations of 5-FU or nanoparticles as 5-FU carriers with and without an iron oxide core for one volume-doubling time of the spheroids (71 h) and hyperthermia at 43 °C for 1 h. Finally, the effect of treatment on viability and level of DNA damage was examined using trypan blue dye exclusion assay and alkaline comet assay, respectively.Results: Results showed that hyperthermia in combination with 5-FU or nanoparticles as 5-FU carriers significantly induced the most DNA damage as compared with the control group. The extent of DNA damage following treatment with 5-FU-loaded nanoparticles combined with hyperthermia was significantly more than for 5-FU combined with hyperthermia. In comparison to the effect of 5-FU-loaded nanoparticles with the iron oxide core and 5-FU-loaded nanoparticle without the iron oxide core, the nanoparticles with the iron oxide core combined with hyperthermia induced more DNA damage than the nanoparticles without the iron oxide core.Conclusions: According to this study, hyperthermia is a harmful agent and nanoparticles are effective delivery vehicles for drugs into colon cancer cells. The iron oxide filled nanoparticles increased the effect of the hyperthermia. All these factors have a significant role in the treatment of colorectal cancer cells. © 2015 Informa UK Ltd. All rights reserved.
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