Theranostic Muc-1 Aptamer Targeted Gold Coated Superparamagnetic Iron Oxide Nanoparticles for Magnetic Resonance Imaging and Photothermal Therapy of Colon Cancer Publisher Pubmed



Azhdarzadeh M^{1, 2} ; Atyabi F^{1, 2} ; Saei AA³ ; Varnamkhasti BS^{1, 2} ; Omidi Y⁴ ; Fateh M⁵ ; Ghavami M⁶ ; Shanehsazzadeh S⁷ ; Dinarvand R^{1, 2} Show Affiliations
Source: Colloids and Surfaces B: Biointerfaces Published:2016

Abstract

Favorable physiochemical properties and the capability to accommodate targeting moieties make superparamegnetic iron oxide nanoparticles (SPIONs) popular theranostic agents. In this study, we engineered SPIONs for magnetic resonance imaging (MRI) and photothermal therapy of colon cancer cells. SPIONs were synthesized by microemulsion method and were then coated with gold to reduce their cytotoxicity and to confer photothermal capabilities. Subsequently, the NPs were conjugated with thiol modified MUC-1 aptamers. The resulting NPs were spherical, monodisperse and about 19 nm in size, as shown by differential light scattering (DLS) and transmission electron microscopy (TEM). UV and X-ray photoelectron spectroscopy (XPS) confirmed the successful gold coating. MTT results showed that Au@SPIONs have insignificant cytotoxicity at the concentration range of 10-100 μg/ml (P > 0.05) and that NPs covered with protein corona exerted lower cytotoxicity than bare NPs. Furthermore, confocal microscopy confirmed the higher uptake of aptamer-Au@SPIONs in comparison with non-targeted SPIONs. MR imaging revealed that SPIONs produced significant contrast enhancement in vitro and they could be exploited as contrast agents. Finally, cells treated with aptamer-Au@SPIONs exhibited a higher death rate compared to control cells upon exposure to near infrared light (NIR). In conclusion, MUC1-aptamer targeted Au@SPIONs could serve as promising theranostic agents for simultaneous MR imaging and photothermal therapy of cancer cells. © 2016 Elsevier B.V.