Evaluating the Antibiotic Resistance and Frequency of Adhesion Markers Among Escherichia Coli Isolated From Type 2 Diabetes Patients With Urinary Tract Infection and Its Association With Common Polymorphism of Mannose-Binding Lectin Gene

Evaluating the Antibiotic Resistance and Frequency of Adhesion Markers Among Escherichia Coli Isolated From Type 2 Diabetes Patients With Urinary Tract Infection and Its Association With Common Polymorphism of Mannose-Binding Lectin Gene Publisher

Mehrabi M¹ ; Salehi B¹ ; Rassi H¹ ; Dehghan A²

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1. Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
2. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: New Microbes and New Infections Published:2020

Abstract

The present paper aims to determine the frequency and antibiotic resistance patterns of pathogenic bacteria, the virulence factor profile of Escherichia coli and mannose-binding lectin (MBL) gene polymorphism in individuals with diabetes mellitus (DM) and urinary tract infection (UTI). The population under study was 130 individuals with type 2 diabetes mellitus (T2DM) and UTI. The patients' clinical characteristics and urine and blood samples (5 mL) were collected. Antibiotic resistance was determined using a disc diffusion method, and the results were interpreted according to CLSI. The presence of virulence genes was detected by multiplex PCR. To detect the MBL gene polymorphism, PCR and restriction fragment length polymorphism methods were applied. The predominant Gram-negative and Gram-positive bacteria included E. coli and Streptococcus spp.viridans group, respectively. Women were more susceptible to the incidence of UTI than men. The E. coli isolates showed a high level of resistance to amoxicillin-clavulanic acid (87.35%), and nitrofurantoin and ceftizoxime were the most effective antimicrobial agents for E. coli. Cefotaxime and ceftizoxime were the most effective antimicrobial agents for Enterobacter spp., norfloxacin and ciprofloxacin were the most effective antimicrobial agents for Staphylococcus epidermidis and Staphylococcus saprophyticus. papGII (52.87%) and papEF (1.14%) had the highest and lowest frequency among examined genes in E. coli isolates, respectively. The GG genotype had the highest frequency among patients with T2DM and UTI. Results showed that the detection of E. coli in individuals with an AA genotype, codon 54 of the MBL gene, can play an important role in the molecular diagnosis and timely treatment of bacterial infections in individuals with diabetes. © 2020 The Authors

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Style	Citing Format
MLA	Mehrabi M, et al.. "Evaluating the Antibiotic Resistance and Frequency of Adhesion Markers Among Escherichia Coli Isolated From Type 2 Diabetes Patients With Urinary Tract Infection and Its Association With Common Polymorphism of Mannose-Binding Lectin Gene." New Microbes and New Infections, vol. 38, no. , 2020, pp. -.
APA	Mehrabi M, Salehi B, Rassi H, Dehghan A (2020). Evaluating the Antibiotic Resistance and Frequency of Adhesion Markers Among Escherichia Coli Isolated From Type 2 Diabetes Patients With Urinary Tract Infection and Its Association With Common Polymorphism of Mannose-Binding Lectin Gene. New Microbes and New Infections, 38(), -.
Chicago	Mehrabi M, Salehi B, Rassi H, Dehghan A. "Evaluating the Antibiotic Resistance and Frequency of Adhesion Markers Among Escherichia Coli Isolated From Type 2 Diabetes Patients With Urinary Tract Infection and Its Association With Common Polymorphism of Mannose-Binding Lectin Gene." New Microbes and New Infections 38, no. (2020): -.
Harvard	Mehrabi M et al. (2020) 'Evaluating the Antibiotic Resistance and Frequency of Adhesion Markers Among Escherichia Coli Isolated From Type 2 Diabetes Patients With Urinary Tract Infection and Its Association With Common Polymorphism of Mannose-Binding Lectin Gene', New Microbes and New Infections, 38(), pp. -.
Vancouver	Mehrabi M, Salehi B, Rassi H, Dehghan A. Evaluating the Antibiotic Resistance and Frequency of Adhesion Markers Among Escherichia Coli Isolated From Type 2 Diabetes Patients With Urinary Tract Infection and Its Association With Common Polymorphism of Mannose-Binding Lectin Gene. New Microbes and New Infections. 2020;38():-.
BibTex	@article{ author = {Mehrabi M and Salehi B and Rassi H and Dehghan A}, title = {Evaluating the Antibiotic Resistance and Frequency of Adhesion Markers Among Escherichia Coli Isolated From Type 2 Diabetes Patients With Urinary Tract Infection and Its Association With Common Polymorphism of Mannose-Binding Lectin Gene}, journal = {New Microbes and New Infections}, volume = {38}, number = {}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Mehrabi M AU - Salehi B AU - Rassi H AU - Dehghan A TI - Evaluating the Antibiotic Resistance and Frequency of Adhesion Markers Among Escherichia Coli Isolated From Type 2 Diabetes Patients With Urinary Tract Infection and Its Association With Common Polymorphism of Mannose-Binding Lectin Gene JO - New Microbes and New Infections VL - 38 IS - SP - EP - PY - 2020 ER -

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