Radiation Therapy Affects the Mechanical Behavior of Human Umbilical Vein Endothelial Cells

Radiation Therapy Affects the Mechanical Behavior of Human Umbilical Vein Endothelial Cells Publisher Pubmed

Mohammadkarim A¹ ; Tabatabaei M² ; Parandakh A² ; Mokhtaridizaji M¹ ; Tafazzolishadpour M² ; Khani MM^{3, 4}

Show Affiliations

1. Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2. Cardiovascular Engineering Lab, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran
3. Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Journal of the Mechanical Behavior of Biomedical Materials Published:2018

Abstract

Radiation therapy has been widely utilized as an effective method to eliminate malignant tumors and cancerous cells. However, subjection of healthy tissues and the related networks of blood vessels adjacent to the tumor area to irradiation is inevitable. The aim of this study was to investigate the consequent effects of fractionation radiotherapy on the mechanical characteristics of human umbilical vein endothelial cells (HUVECs) through alterations in cytoskeleton organization and cell and nucleus morphology. In order to simulate the clinical condition of radiotherapy, the HUVECs were exposed to the specific dose of 2 Gy for 1–4 times among four groups with incremental total dose from 2 Gy up to 8 Gy. Fluorescence staining was performed to label F-actin filaments and nuclei. Micropipette aspiration and standard linear solid model were employed to evaluate the elastic and viscoelastic characteristics of the HUVECs. Radiotherapy significantly increased cell elastic moduli. Due to irradiation, instantaneous and equilibrium Young's modulus were also increased. Radiotherapy diminished HUVECs viscoelastic behavior and shifted their creep compliance curves downward. Furthermore, gamma irradiation elevated the nuclei sizes and to a lesser extent the cells sizes resulting in the accumulation of F-actin filaments within the rest of cell body. Endothelial stiffening correlates with endothelial dysfunction, hence the results may be helpful when the consequent effects of radiotherapy are the focus of concern. © 2018

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Experts (# of related papers)

Ali Kazemian (3)

Style	Citing Format
MLA	Mohammadkarim A, et al.. "Radiation Therapy Affects the Mechanical Behavior of Human Umbilical Vein Endothelial Cells." Journal of the Mechanical Behavior of Biomedical Materials, vol. 85, no. , 2018, pp. 188-193.
APA	Mohammadkarim A, Tabatabaei M, Parandakh A, Mokhtaridizaji M, Tafazzolishadpour M, Khani MM (2018). Radiation Therapy Affects the Mechanical Behavior of Human Umbilical Vein Endothelial Cells. Journal of the Mechanical Behavior of Biomedical Materials, 85(), 188-193.
Chicago	Mohammadkarim A, Tabatabaei M, Parandakh A, Mokhtaridizaji M, Tafazzolishadpour M, Khani MM. "Radiation Therapy Affects the Mechanical Behavior of Human Umbilical Vein Endothelial Cells." Journal of the Mechanical Behavior of Biomedical Materials 85, no. (2018): 188-193.
Harvard	Mohammadkarim A et al. (2018) 'Radiation Therapy Affects the Mechanical Behavior of Human Umbilical Vein Endothelial Cells', Journal of the Mechanical Behavior of Biomedical Materials, 85(), pp. 188-193.
Vancouver	Mohammadkarim A, Tabatabaei M, Parandakh A, Mokhtaridizaji M, Tafazzolishadpour M, Khani MM. Radiation Therapy Affects the Mechanical Behavior of Human Umbilical Vein Endothelial Cells. Journal of the Mechanical Behavior of Biomedical Materials. 2018;85():188-193.
BibTex	@article{ author = {Mohammadkarim A and Tabatabaei M and Parandakh A and Mokhtaridizaji M and Tafazzolishadpour M and Khani MM}, title = {Radiation Therapy Affects the Mechanical Behavior of Human Umbilical Vein Endothelial Cells}, journal = {Journal of the Mechanical Behavior of Biomedical Materials}, volume = {85}, number = {}, pages = {188-193}, year = {2018} }
RIS	TY - JOUR AU - Mohammadkarim A AU - Tabatabaei M AU - Parandakh A AU - Mokhtaridizaji M AU - Tafazzolishadpour M AU - Khani MM TI - Radiation Therapy Affects the Mechanical Behavior of Human Umbilical Vein Endothelial Cells JO - Journal of the Mechanical Behavior of Biomedical Materials VL - 85 IS - SP - 188 EP - 193 PY - 2018 ER -

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