The Mechanical Characteristics of Human Endothelial Cells in Response to Single Ionizing Radiation Doses by Using Micropipette Aspiration Technique

The Mechanical Characteristics of Human Endothelial Cells in Response to Single Ionizing Radiation Doses by Using Micropipette Aspiration Technique Publisher

Mohammadkarim A^{1, 2, 3} ; Mokhtaridizaji M^{1, 3} ; Kazemian A⁴ ; Saberi H⁵ ; Khani MM⁶ ; Bakhshandeh M⁷

Show Affiliations

1. Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2. Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran
3. Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4. Radiation Oncology Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran
5. Department of Radiology, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran
6. Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7. Radiology Technology Department, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: MCB Molecular and Cellular Biomechanics Published:2019

Abstract

The mechanical properties of living cells are known to be promising biomarkers when investigating the health and functions of the human body. Ionizing irradiation results in vascular injury due to endothelial damage. Thus, the current study objective was to evaluate the influence of continuous radiation doses on the mechanical properties of human umbilical vein endothelial cells (HUVECs), and to identify Young's modulus (E) and viscoelastic behavior. Single-dose (0, 2, 4, 6, and 8 Gy) radiation was applied to HUVECs using a Cobalt-60 treatment machine in the current vitro irradiation study. Thereafter, a micropipette-aspiration technique was used to measure the elastic modulus of the HUVECs in control and radiation-induced samples. Confocal imaging was then performed for following of the cytoskeletal reorganization of the HUVECs in response to the different radiation doses. Significant enhanced adhesion of the elastic modulus of the HUVECs was observed. The dose value was seen to increase from 0 Gy to 8 Gy. A linear relationship was observed between the 0 Gy and 8 Gy doses following an examination of the dose-response curve for elastic modulus after irradiation. The correlation coefficient was found to be 0.955 and the sensitivity of the dose-elastic modulus to be 7.69 Pa..Gy-1 following analysis of the linear portion of the response curve. Also, a significant increment in stiffness accompanied with the considerable drop in creep compliance curve was detected in radiation-induced groups. Biomechanics-based analysis can provide a platform from which to assess the response of the endothelium to radiation when studying vascular system behavior during the cancer therapy process. © 2019 Tech Science Press. All rights reserved.

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Experts (# of related papers)

Ali Kazemian (3)

Niloofar Ayoobi Yazdi (1)

Style	Citing Format
MLA	Mohammadkarim A, et al.. "The Mechanical Characteristics of Human Endothelial Cells in Response to Single Ionizing Radiation Doses by Using Micropipette Aspiration Technique." MCB Molecular and Cellular Biomechanics, vol. 16, no. 4, 2019, pp. 275-287.
APA	Mohammadkarim A, Mokhtaridizaji M, Kazemian A, Saberi H, Khani MM, Bakhshandeh M (2019). The Mechanical Characteristics of Human Endothelial Cells in Response to Single Ionizing Radiation Doses by Using Micropipette Aspiration Technique. MCB Molecular and Cellular Biomechanics, 16(4), 275-287.
Chicago	Mohammadkarim A, Mokhtaridizaji M, Kazemian A, Saberi H, Khani MM, Bakhshandeh M. "The Mechanical Characteristics of Human Endothelial Cells in Response to Single Ionizing Radiation Doses by Using Micropipette Aspiration Technique." MCB Molecular and Cellular Biomechanics 16, no. 4 (2019): 275-287.
Harvard	Mohammadkarim A et al. (2019) 'The Mechanical Characteristics of Human Endothelial Cells in Response to Single Ionizing Radiation Doses by Using Micropipette Aspiration Technique', MCB Molecular and Cellular Biomechanics, 16(4), pp. 275-287.
Vancouver	Mohammadkarim A, Mokhtaridizaji M, Kazemian A, Saberi H, Khani MM, Bakhshandeh M. The Mechanical Characteristics of Human Endothelial Cells in Response to Single Ionizing Radiation Doses by Using Micropipette Aspiration Technique. MCB Molecular and Cellular Biomechanics. 2019;16(4):275-287.
BibTex	@article{ author = {Mohammadkarim A and Mokhtaridizaji M and Kazemian A and Saberi H and Khani MM and Bakhshandeh M}, title = {The Mechanical Characteristics of Human Endothelial Cells in Response to Single Ionizing Radiation Doses by Using Micropipette Aspiration Technique}, journal = {MCB Molecular and Cellular Biomechanics}, volume = {16}, number = {4}, pages = {275-287}, year = {2019} }
RIS	TY - JOUR AU - Mohammadkarim A AU - Mokhtaridizaji M AU - Kazemian A AU - Saberi H AU - Khani MM AU - Bakhshandeh M TI - The Mechanical Characteristics of Human Endothelial Cells in Response to Single Ionizing Radiation Doses by Using Micropipette Aspiration Technique JO - MCB Molecular and Cellular Biomechanics VL - 16 IS - 4 SP - 275 EP - 287 PY - 2019 ER -

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