Correlation of the Cell Mechanical Behavior and Quantified Cytoskeletal Parameters in Normal and Cancerous Breast Cell Lines

Correlation of the Cell Mechanical Behavior and Quantified Cytoskeletal Parameters in Normal and Cancerous Breast Cell Lines Publisher Pubmed

Tabatabaei M¹ ; Tafazzolishadpour M¹ ; Khani MM²

Show Affiliations

1. Cardiovascular Engineering Lab, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran Polytechnic, Tehran, Iran
2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Biorheology Published:2019

Abstract

BACKGROUND: Cancerous transformation of cells affects their mechanical behavior and cytoskeleton structure. OBJECTIVE: The objective of this research is to investigate a correlation between mechanical properties and cytoskeletal structure features in cancer cell formation. METHODS: Micropipette aspiration was used to compare mechanical properties of normal (MCF10A) and cancerous (T47D) epithelial breast cell lines. Immunofluorescence and confocal microscopy were employed for staining and imaging F-actin and microtubules, and quantifying their fluorescent intensity, anisotropy and fiber distribution. RESULTS: Results indicated higher F-actin intensity (43%) and anisotropy (50%) in normal cells compared to cancer cells, although there was no difference in the microtubules intensity between cell lines. Furthermore, reductions of cortex thickness and actin layer index (60%) were observed in suspended cancer cells compared to normal cells. Changes in cell physical properties induced by cancer were attributed to microtubules. The arranged fibrous structure of microtubules in normal cells was replaced by a disorganized structure in cancer cells. Cancerous cells were about four times softer with higher creep compliance compared to normal cells. CONCLUSIONS: Results of this study confirmed that alterations in cell mechanical properties induced by cancer are highly correlated with changes in F-actin and microtubule content and arrangement. It is suggested that such changes can enhance our knowledge of cancer initiation and progression. © 2019 IOS Press and the authors. All rights reserved.

Related Docs

View other Related Docs

1. Mechanical Characterization of Human Mesenchymal Stem Cells Subjected to Cyclic Uniaxial Strain and Tgf-Β1, Journal of the Mechanical Behavior of Biomedical Materials (2015)

2. The Effects of Short-Term Uniaxial Strain on the Mechanical Properties of Mesenchymal Stem Cells Upon Tgf-Β1 Stimulation, In Vitro Cellular and Developmental Biology - Animal (2018)

3. Substrate Topography Interacts With Substrate Stiffness and Culture Time to Regulate Mechanical Properties and Smooth Muscle Differentiation of Mesenchymal Stem Cells, Colloids and Surfaces B: Biointerfaces (2019)

4. Radiation Therapy Affects the Mechanical Behavior of Human Umbilical Vein Endothelial Cells, Journal of the Mechanical Behavior of Biomedical Materials (2018)

5. Quantification of Effects of Cancer on Elastic Properties of Breast Tissue by Atomic Force Microscopy, Journal of the Mechanical Behavior of Biomedical Materials (2016)

6. The Mechanical Characteristics of Human Endothelial Cells in Response to Single Ionizing Radiation Doses by Using Micropipette Aspiration Technique, MCB Molecular and Cellular Biomechanics (2019)

7. An Afm-Based Nanomechanical Study of Ovarian Tissues With Pathological Conditions, International Journal of Nanomedicine (2020)

8. Dose-Dependent 60Co Γ-Radiation Effects on Human Endothelial Cell Mechanical Properties, Cell Biochemistry and Biophysics (2019)

Experts (# of related papers)

View all Related Experts

Ali Kazemian (2)

Fatemeh Nili Ahmadabadi (1)

Style	Citing Format
MLA	Tabatabaei M, Tafazzolishadpour M, Khani MM. "Correlation of the Cell Mechanical Behavior and Quantified Cytoskeletal Parameters in Normal and Cancerous Breast Cell Lines." Biorheology, vol. 56, no. 4, 2019, pp. 207-219.
APA	Tabatabaei M, Tafazzolishadpour M, Khani MM (2019). Correlation of the Cell Mechanical Behavior and Quantified Cytoskeletal Parameters in Normal and Cancerous Breast Cell Lines. Biorheology, 56(4), 207-219.
Chicago	Tabatabaei M, Tafazzolishadpour M, Khani MM. "Correlation of the Cell Mechanical Behavior and Quantified Cytoskeletal Parameters in Normal and Cancerous Breast Cell Lines." Biorheology 56, no. 4 (2019): 207-219.
Harvard	Tabatabaei M, Tafazzolishadpour M, Khani MM (2019) 'Correlation of the Cell Mechanical Behavior and Quantified Cytoskeletal Parameters in Normal and Cancerous Breast Cell Lines', Biorheology, 56(4), pp. 207-219.
Vancouver	Tabatabaei M, Tafazzolishadpour M, Khani MM. Correlation of the Cell Mechanical Behavior and Quantified Cytoskeletal Parameters in Normal and Cancerous Breast Cell Lines. Biorheology. 2019;56(4):207-219.
BibTex	@article{ author = {Tabatabaei M and Tafazzolishadpour M and Khani MM}, title = {Correlation of the Cell Mechanical Behavior and Quantified Cytoskeletal Parameters in Normal and Cancerous Breast Cell Lines}, journal = {Biorheology}, volume = {56}, number = {4}, pages = {207-219}, year = {2019} }
RIS	TY - JOUR AU - Tabatabaei M AU - Tafazzolishadpour M AU - Khani MM TI - Correlation of the Cell Mechanical Behavior and Quantified Cytoskeletal Parameters in Normal and Cancerous Breast Cell Lines JO - Biorheology VL - 56 IS - 4 SP - 207 EP - 219 PY - 2019 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract