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Rna-Sequencing for Transcriptional Profiling of Whole Blood in Early Stage and Metastatic Pancreatic Cancer Patients Publisher Pubmed



Kalantari S1 ; Kazemi B1 ; Roudi R2 ; Zali H3, 4 ; Dangelo A5 ; Mohamadkhani A6 ; Madjd Z7 ; Pourshams A6, 8
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Authors Affiliations
  1. 1. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Department of Medicine, University of Minnesota Medical School, Minneapolis, MN, United States
  3. 3. Proteomics Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran
  4. 4. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom
  6. 6. Liver and Pancreatobiliary Diseases Research Center, Digestive Disease Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Oncopathology Research Center, Iran University of Medical Sciences, Tehran, Iran
  8. 8. Digestive Oncology Research Center, Digestive Diseases Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

Source: Cell Biology International Published:2023


Abstract

We investigated the transcriptional profile of whole blood in early and metastatic stages of pancreatic cancer (PaC) patients to identify potential diagnostic factors for early diagnosis. Blood samples from 18 participants (6 healthy individuals, 6 patients in early stage (I/II) PaC, and 6 patients in metastatic PaC) were analyzed by RNA-sequencing. The expression levels of identified genes were subsequently compared with their expression in pancreatic tumor tissues based on TCGA data reported in UALCAN and GEPIA2 databases. Overall, 331 and 724 genes were identified as differentially expressed genes in early and metastatic stages, respectively. Of these, 146 genes were shared by early and metastatic stages. Upregulation of PTCD3 and UBA52 genes and downregulation of A2M and ARID1B genes in PaC patients were observed from early stage to metastasis. TCGA database showed increasing trend in expression levels of these genes from stage I to IV in pancreatic tumor tissue. Finally, we found that low expression of PTCD3, A2M, and ARID1B genes and high expression of UBA52 gene were positively correlated with PaC patients survival. We identified a four-gene set (PTCD3, UBA52, A2M, and ARID1B) expressed in peripheral blood of early stage and metastatic PaC patients that may be useful for PaC early diagnosis. © 2022 International Federation for Cell Biology.
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