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Differential Expression of Two Genes Oct-4 and Muc5ac Associates With Poor Outcome in Patients With Gastric Cancer Publisher Pubmed



Javanbakht M1 ; Akhavanmoghadam J2 ; Talaei AJ3 ; Aghyani M4 ; Mozafari M4, 5 ; Khedmat L6 ; Mohebbi M4
Authors
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Authors Affiliations
  1. 1. School of Medicine Science, Islamic Azad University, Sarab, Iran
  2. 2. Trauma Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR, Iran
  3. 3. Department of Genetics, Faculty of Life Science, Azad University of Tehran Medical Sciences Branch, Tehran, Iran
  4. 4. General practitioner (GP), Doctor of Medicine (MD), Tehran and Tabriz, Iran
  5. 5. Graduated from Tabriz University of Medical Science, Tabriz, Iran
  6. 6. Department of Social Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Clinical and Experimental Pharmacology and Physiology Published:2017


Abstract

Gastric cancer (GC) is the most frequent leading cause of cancer-associated mortality worldwide that is linked to poor prognosis due to the lack of appropriate biomarkers. Our aim was to evaluate the MUC5AC and Oct-4 expression levels in GC and to assess their association with clinical factors. Immunohistochemical analysis (IHC) and qRT-PCR were performed in GC patients to examine the MUC5AC and Oct-4 expression levels. The mRNA level of MUC5AC was significantly decreased in tumour tissues compared with non-cancerous tissues (1.11 ± 0.69 vs 3.7 ± 0.71; P =.024). On the other hand, Oct-4 mRNA level was upregulated in tumour tissues as compared to normal tissues (2. 86 ± 0.78 vs 0.87 ± 0.54; P =.0015). Decreased expression of MUC5AC was detected in 27 patients (67.5%), while high to moderate expression levels were observed in 13 cases (32.5%), but in normal tissues the expression levels of MUC5AC were increased (P =.001). The decreased expression of MUC5AC was associated with aggressive tumour characteristics, such as TNM stage (P =.023), histologic type (P =.012) and lymph node metastasis (P =.001). High expression of Oct-4 was detected in 24 tumour tissues (60%), while 16 cases (40%) showed low expression level. Increased Oct-4 expression was correlated with clinicopathological characteristics such TNM stage (P =.002), histologic type (P =.008) and lymph node metastasis (P =.001). Our results showed that high Oct-4 expression and the reduction of MUC5AC expression may be involved in the progression and an unfavorable prognosis of GC. © 2017 John Wiley & Sons Australia, Ltd
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