Simultaneous Formulation of Influenza Vaccine and Chitosan Nanoparticles Within Cpg Oligodesoxi Nucleotides Leads to Dose-Sparing and Protects Against Lethal Challenge in the Mouse Model

Simultaneous Formulation of Influenza Vaccine and Chitosan Nanoparticles Within Cpg Oligodesoxi Nucleotides Leads to Dose-Sparing and Protects Against Lethal Challenge in the Mouse Model Publisher Pubmed

Sadati SF^{1, 2} ; Jamali A³ ; Abdoli A⁴ ; Abedivalugerdi M⁵ ; Gholami S⁶ ; Alipour S⁷ ; Soleymani S⁴ ; Kheiri MT³ ; Atyabi F⁸

Show Affiliations

1. Department of Medical Microbiology, Ondokuz Mayis University Medical School, Samsun, Turkey
2. Amasya University Research Laboratory Center, Ipekkoy Campus, Amasya, Turkey
3. Influenza Unit, Pasteur Institute of Iran, Tehran, Iran
4. Department of Hepatitis and AIDS, Pasteur Institute of Iran, No. 69, Pasteur Avenue, Tehran, Iran
5. Experimentell Cancer Medicin (ECM), Halsovagen Huddinge, Sweden
6. Department of Pharmaceutical Biotechnology, Pasteur Institute of Iran, Tehran, Iran
7. Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, 45195-1159, Iran
8. Department of Pharmaceutical Nanotechnology, Tehran University of Medical Sciences, Tehran, Iran

Source: Pathogens and Disease Published:2018

Abstract

Lack of efficient delivery systems for transporting antigenic molecules to the cytosol of antigen-presenting cells presents a major obstacle for antigen uptake by immune cells. To this end, influenza whole inactivated virus vaccines were formulated with chitosan nanoparticles and CpG oligonucleotide as a biodegradable delivery system and a Th1-specific adjuvant, respectively. Intradermal injections of a single high dose and low dose of formulated candidate vaccines were carried out. Thirty days after injection, cell proliferation assay (MTT), IFN-gamma and IL-4 ELISpot assays were conducted. Sera samples were collected 21 days after immunization to measure IgG1 and IgG2a levels. In addition, the mice challenged with mouse-adopted virus were monitored for weight loss. The results show a significant stimulation of both humoral and cellular immunities; also, weight gain and a decrease in mortality in the mice receiving both dosages of inactivated influenza virus vaccines with CpG and Chitosan coating were observed. Based on the results, it can be concluded that formulation of inactivated influenza virus with CpG and its delivery by chitosan as low-dose can return the same results as with high-dose balanced between cellular and humeral immune responses. This formulation could potentially lead to a significant saving in vaccine production. © 2018 FEMS.

2. Chitosan As a Machine for Biomolecule Delivery: A Review, Carbohydrate Polymers (2021)

3. Fast Antibody Responses by Immuno-Targeting and Nanotechnology Strategies Versus Hbsag Vaccine, Iranian Journal of Basic Medical Sciences (2021)

Style	Citing Format
MLA	Sadati SF, et al.. "Simultaneous Formulation of Influenza Vaccine and Chitosan Nanoparticles Within Cpg Oligodesoxi Nucleotides Leads to Dose-Sparing and Protects Against Lethal Challenge in the Mouse Model." Pathogens and Disease, vol. 76, no. 8, 2018, pp. -.
APA	Sadati SF, Jamali A, Abdoli A, Abedivalugerdi M, Gholami S, Alipour S, Soleymani S, Kheiri MT, Atyabi F (2018). Simultaneous Formulation of Influenza Vaccine and Chitosan Nanoparticles Within Cpg Oligodesoxi Nucleotides Leads to Dose-Sparing and Protects Against Lethal Challenge in the Mouse Model. Pathogens and Disease, 76(8), -.
Chicago	Sadati SF, Jamali A, Abdoli A, Abedivalugerdi M, Gholami S, Alipour S, Soleymani S, Kheiri MT, Atyabi F. "Simultaneous Formulation of Influenza Vaccine and Chitosan Nanoparticles Within Cpg Oligodesoxi Nucleotides Leads to Dose-Sparing and Protects Against Lethal Challenge in the Mouse Model." Pathogens and Disease 76, no. 8 (2018): -.
Harvard	Sadati SF et al. (2018) 'Simultaneous Formulation of Influenza Vaccine and Chitosan Nanoparticles Within Cpg Oligodesoxi Nucleotides Leads to Dose-Sparing and Protects Against Lethal Challenge in the Mouse Model', Pathogens and Disease, 76(8), pp. -.
Vancouver	Sadati SF, Jamali A, Abdoli A, Abedivalugerdi M, Gholami S, Alipour S, et al.. Simultaneous Formulation of Influenza Vaccine and Chitosan Nanoparticles Within Cpg Oligodesoxi Nucleotides Leads to Dose-Sparing and Protects Against Lethal Challenge in the Mouse Model. Pathogens and Disease. 2018;76(8):-.
BibTex	@article{ author = {Sadati SF and Jamali A and Abdoli A and Abedivalugerdi M and Gholami S and Alipour S and Soleymani S and Kheiri MT and Atyabi F}, title = {Simultaneous Formulation of Influenza Vaccine and Chitosan Nanoparticles Within Cpg Oligodesoxi Nucleotides Leads to Dose-Sparing and Protects Against Lethal Challenge in the Mouse Model}, journal = {Pathogens and Disease}, volume = {76}, number = {8}, pages = {-}, year = {2018} }
RIS	TY - JOUR AU - Sadati SF AU - Jamali A AU - Abdoli A AU - Abedivalugerdi M AU - Gholami S AU - Alipour S AU - Soleymani S AU - Kheiri MT AU - Atyabi F TI - Simultaneous Formulation of Influenza Vaccine and Chitosan Nanoparticles Within Cpg Oligodesoxi Nucleotides Leads to Dose-Sparing and Protects Against Lethal Challenge in the Mouse Model JO - Pathogens and Disease VL - 76 IS - 8 SP - EP - PY - 2018 ER -

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