Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Tumor Suppressive Function of Microrna-192 in Acute Lymphoblastic Leukemia Publisher Pubmed



Sayadi M1 ; Ajdary S2 ; Nadali F1 ; Rostami S3 ; Fahtabad ME1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Hematology, Allied Medical School, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
  3. 3. Department of Hematology, Hematology and Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Bosnian Journal of Basic Medical Sciences Published:2017


Abstract

Non-coding RNAs play a critical role in gene regulation in cancer cells. Reduced expression of microRNA-192 (miR-192) has been detected in many cancers. In this study, we investigated the role of miR-192 in cell proliferation and cell cycle control in NALM-6 cell line, a model of acute lymphoblastic leukemia (ALL). Cell cycle analysis by DNA content using propidium iodide staining and cell apoptosis analysis using Annexin V assay were carried out. Cell proliferation changes were monitored using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. In addition, the relative changes in P53, BAX, CASP3, and BCL-2 gene expression were determined by quantitative reverse transcription PCR. Overexpression of miR-192 resulted in cell proliferation arrest in ALL cells. After 72 and 96 hours of transduction, apoptosis was significantly increased in the cells transduced with miR-192-overexpressing virus compared with control cells. The expression of P53, BAX, and CASP3 increased after 48 hours of transduction in miR-192-overexpressing cells, but no change was observed in BCL-2 expression. The G0/S and G1/S ratio changed to 7.5 and 4.5, respectively, in the cells overexpressing miR-192 compared with controls. The results of our study suggest, for the first time, tumor suppressive effects of miR-192 in ALL cells. © 2017 ABMSFBIH.
Related Docs
View other Related Docs
1. Micrornas As Biomarkers for Early Diagnosis, Targeting and Prognosis of Prostate Cancer, Pathology Research and Practice (2023)
2. Serum and Tissue Mirnas: Potential Biomarkers for the Diagnosis of Cervical Cancer, Virology Journal (2019)
3. Circulating Levels of the Mirnas, Mir-194, and Mir-29B, As Clinically Useful Biomarkers for Colorectal Cancer, Tumor Biology (2016)
4. The Role of Bax in the Apoptosis of Leishmania-Infected Macrophages, Microbial Pathogenesis (2020)
5. Deregulation of Mir-1, Mir486, and Let-7A in Cytogenetically Normal Acute Myeloid Leukemia: Association With Npm1 and Flt3 Mutation and Clinical Characteristics, Tumor Biology (2016)
6. Cell Death Pathways and Viruses: Role of Micrornas, Molecular Therapy Nucleic Acids (2021)
7. Micrornas (Mirs) Precisely Regulate Immune System Development and Function in Immunosenescence Process, International Reviews of Immunology (2016)
8. The Intricate Role of Mir-155 in Carcinogenesis: Potential Implications for Esophageal Cancer Research, Biomarkers in Medicine (2019)
Experts (# of related papers)
View all Related Experts
Hamid Reza Mirzaei (4)
Nima Rezaei (3)
Other Related Docs
9. Upregulation of Mir-371-373 Cluster, a Human Embryonic Stem Cell Specific Microrna Cluster, in Esophageal Squamous Cell Carcinoma, Journal of Cancer Research and Therapeutics (2018)
10. Circulating Micrornas in Hepatocellular Carcinoma: Potential Diagnostic and Prognostic Biomarkers, Current Pharmaceutical Design (2016)
11. The Possible Effect of Microrna-155 (Mir-155) and Bace1 Inhibitors in the Memory of Patients With Down Syndrome and Alzheimer's Disease: Design, Synthesis, Virtual Screening, Molecular Modeling and Biological Evaluations, Journal of Biomolecular Structure and Dynamics (2022)
12. Oncogenomic Changes in Pancreatic Cancer and Their Detection in Stool, Biomolecules (2022)
13. Microrna Signature of Regulatory T Cells in Health and Autoimmunity, Biomedicine and Pharmacotherapy (2018)
14. Testicular Mirnas in Relation to Spermatogenesis, Spermatogonial Stem Cells and Cancer/Testis Genes, Scientific African (2019)
15. Non-Coding Rnas and Glioma: Focus on Cancer Stem Cells, Molecular Therapy Oncolytics (2022)
16. Decreased Expression of Fecal Mir-4478 and Mir-1295B-3P in Early-Stage Colorectal Cancer, Cancer Biomarkers (2015)
17. Microfluidics for Detection of Exosomes and Micrornas in Cancer: State of the Art, Molecular Therapy Nucleic Acids (2022)
18. The Role of Micrornas in Myeloproliferative Neoplasia, International Journal of Hematology-Oncology and Stem Cell Research (2016)
19. Micrornas and Long Non-Coding Rnas As Biomarkers for Polycystic Ovary Syndrome, Journal of Cellular and Molecular Medicine (2022)
20. Downregulation of Plasma Mir-142-3P and Mir-26A-5P in Patients With Colorectal Carcinoma, International Journal of Cancer Management (2015)
21. Restoration of Mir-648 Overcomes 5-Fu-Resistance Through Targeting Et-1 in Gastric Cancer Cells In-Vitro, Pathology Research and Practice (2022)
22. Exosomal Micrornas in Regulation of Tumor Cells Resistance to Apoptosis, Biochemistry and Biophysics Reports (2024)
23. Microrna Profiling During Germline Differentiation of Mouse Embryonic Stem Cells, Cellular and Molecular Biology (2015)
24. Decreased Expression of Bioinformatically Predicted Piwil2-Targetting Micrornas, Mir-1267 and Mir-2276 in Breast Cancer, Archives of Iranian Medicine (2016)
25. Identification of Candidate Microrna Markers of Endometriosis With the Use of Next-Generation Sequencing and Quantitative Real-Time Polymerase Chain Reaction, Fertility and Sterility (2020)
26. Exosomes and Micrornas in Biomedical Science, Synthesis Lectures on Biomedical Engineering (2022)
27. Role of Micrornas in Chronic Lymphocytic Leukemia Pathogenesis, Current Medicinal Chemistry (2020)
28. The Role of Micrornas in the Viral Infections, Current Pharmaceutical Design (2018)
29. The Effects of Oxygen–Ozone Therapy on Regulatory T-Cell Responses in Multiple Sclerosis Patients, Cell Biology International (2021)
30. Microrna Machinery in Parkinson’S Disease: A Platform for Neurodegenerative Diseases, Expert Review of Neurotherapeutics (2022)