Mir-192 Overexpression Effect on Dhfr and Tyms in Acute Lymphoblastic Leukemia

Mir-192 Overexpression Effect on Dhfr and Tyms in Acute Lymphoblastic Leukemia Publisher

Sayadi M¹ ; Rostami S² ; Nadali F¹ ; Fathabad ME^{3, 4} ; Riazirad F⁵ ; Sayadi K⁶ ; Ajdary S⁵

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1. Hematology Department, Allied Medical School, Tehran University of Medical Sciences, Ghods Ave., Tehran, Iran
2. Hematology and Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Kargar Ave., Tehran, Iran
3. Department of Laboratory Sciences, Paramedical Faculty, Tabriz University of Medical Sciences, Daneshgah Ave., Tabriz, Iran
4. Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Daneshgah Ave., Tabriz, Iran
5. Department of Immunology, Pasteur Institute of Iran, 69 Pasteur Ave., Tehran, 13169-43551, Iran
6. Department of Clinical Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Source: Gene Reports Published:2020

Abstract

Purpose: Non-coding RNAs play a critical role in gene regulation in cancer cells. Reduced expression of microRNA-192 has been detected in many cancers. Here, we investigated the role of miR-192 in Dihydrofolate reductase (DHFR) and Thymidylate Synthase (TYMS) expression level and in an acute lymphoblastic leukemia cell line. Basic procedures: 20 patients diagnosed with acute lymphoblastic leukemia (ALL) were studied for the level of (micro RNA-192) miR-192, DHFR and TYMS expression level by qRT-PCR. NALM-6 cells were transduced using recombinant lentiviruses for overexpression of miR-192 and its backbone, then the relative changes in DHFR and TYMS genes expression were studied by qRT-PCR. DHFR Protein level changes were analyzed by Western blotting. Main findings: ALL patients with relapse, experience lower levels of miR-192 and higher levels of DHFR and TYMS in comparison with treated patients. Overexpression of miR-192 in NALM-6 cells resulted in decreased expression of DHFR and TYMS. Moreover, the protein level of DHFR was decreased after overexpression of miR-192. Principal conclusions: These results suggest the tumor suppression effects of miR-192 and its correlation with decreased DHFR and TYMS level in acute lymphoblastic leukemia cells for the first time. © 2020

Style	Citing Format
MLA	Sayadi M, et al.. "Mir-192 Overexpression Effect on Dhfr and Tyms in Acute Lymphoblastic Leukemia." Gene Reports, vol. 21, no. , 2020, pp. -.
APA	Sayadi M, Rostami S, Nadali F, Fathabad ME, Riazirad F, Sayadi K, Ajdary S (2020). Mir-192 Overexpression Effect on Dhfr and Tyms in Acute Lymphoblastic Leukemia. Gene Reports, 21(), -.
Chicago	Sayadi M, Rostami S, Nadali F, Fathabad ME, Riazirad F, Sayadi K, Ajdary S. "Mir-192 Overexpression Effect on Dhfr and Tyms in Acute Lymphoblastic Leukemia." Gene Reports 21, no. (2020): -.
Harvard	Sayadi M et al. (2020) 'Mir-192 Overexpression Effect on Dhfr and Tyms in Acute Lymphoblastic Leukemia', Gene Reports, 21(), pp. -.
Vancouver	Sayadi M, Rostami S, Nadali F, Fathabad ME, Riazirad F, Sayadi K, et al.. Mir-192 Overexpression Effect on Dhfr and Tyms in Acute Lymphoblastic Leukemia. Gene Reports. 2020;21():-.
BibTex	@article{ author = {Sayadi M and Rostami S and Nadali F and Fathabad ME and Riazirad F and Sayadi K and Ajdary S}, title = {Mir-192 Overexpression Effect on Dhfr and Tyms in Acute Lymphoblastic Leukemia}, journal = {Gene Reports}, volume = {21}, number = {}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Sayadi M AU - Rostami S AU - Nadali F AU - Fathabad ME AU - Riazirad F AU - Sayadi K AU - Ajdary S TI - Mir-192 Overexpression Effect on Dhfr and Tyms in Acute Lymphoblastic Leukemia JO - Gene Reports VL - 21 IS - SP - EP - PY - 2020 ER -

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