Characterization of Occult Hepatitis B Infection Among Iranian Liver Transplant Recipients Publisher Pubmed



Khamseh A^{1, 2} ; Poortahmasebi V^{1, 3, 4} ; Soltani S^{1, 2} ; Nasiritoosi M⁵ ; Jafarian A² ; Ghaziasadi A^{1, 2} ; Norouzi M^{1, 2} ; Ghorbani S^{1, 6} ; Eslami N^{3, 4} ; Jazayeri SM^{1, 2, 6} Show Affiliations
Source: Journal of Clinical Laboratory Analysis Published:2022

Abstract

Background: The prevalence of occult hepatitis B infection (OBI) among Iranian liver transplant recipient patients has not been explored yet. The present study aimed to determine the OBI prevalence among Iranian liver transplant recipients. Methods: This study encompassed 97 patients having undergone liver transplantation due to several clinical backgrounds in the Liver Transplantation Center, Tehran, Iran. After serological evaluation, two different types of PCR methods were applied for amplification of HBV DNA, followed by the direct sequencing of whole hepatitis B virus (HBV) surface genes. Results: At the time of admission, none of the patients were positive for HBsAg. However, 24 (25%), 12 (12.3%), and 5 (5.1%) cases were positive for anti-HBc, hepatitis C virus (HCV), and hepatitis delta virus (HDV) antibodies, respectively. Moreover, two males were positive for OBI (2.1%). Both were positive for anti-HBc and negative for anti-HBs, anti-HCV, and anti-HDV. HBV-related cirrhosis was the underlying reason for their liver transplantation. HBsAg sequences revealed no amino acid substitution. Conclusions: The prevalence of OBI in the Iranian liver transplantation patients was relatively low. Future longitudinal studies with a larger sample size are suggested to explore the significance of this clinical finding, including the reactivation of cryptic HBV DNA, in liver transplant subjects. © 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.