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In Vitro and in Vivo Study of Carboxymethyl Chitosan/Polyvinyl Alcohol for Wound Dressing Application Publisher



Akbari A1 ; Rabbani S2 ; Irani S1 ; Zandi M3 ; Sharifi F4 ; Ameli F5 ; Mohamadali M1
Authors
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Authors Affiliations
  1. 1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
  2. 2. Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Biomaterial, Iran Polymer and Petrochemical Institute, Tehran, Iran
  4. 4. Hard Tissue Engineering Research Center, Tissue Engineering and Regenerative Medicine Institute, Central Tehran Branch, Islamic Azad University, Tehran, Iran
  5. 5. Department of Pathology, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Science, Tehran, Iran

Source: Journal of Applied Polymer Science Published:2022


Abstract

Antibacterial, good biosafety, and wound healing enhancers, are the most favorable factors for wound dressing. Designing nanomaterial of the scaffold for supporting skin regeneration is a complicated process. Carboxymethyl chitosan (CMC) structure similar to the glycosaminoglycan and antibacterial activity combined with polyvinyl alcohol (PVA) (PVA/CMC) can lead to satisfactory properties for wound management. In this study, a collection of electrospun nanofibrous scaffolds are fabricated by blending the constant PVA concentration (9%) with a limited area of CMC concentration (2%, 3%, 4%, and 5%). PVA/CMC scaffolds characteristic was investigated through a scanning electron microscope, Fourier-transform infrared spectroscopy, and contact angle tests. The fiber diameter values were measured for PVA (144.60 nm), chitosan (131.57 nm), and average different concentration of CMC in PVA/CMC (104.7 ± 2.45 nm). In vitro potential of the scaffolds were evaluated by using human dermal fibroblast cells and human placental-derived mesenchymal stem cells. According to in vitro results, PVA/CMC4% scaffold was selected as the optimized wound dressing for skin tissue engineering. The in vivo wound healing data in rat models illustrated more healing capacity of PVA/CMC4% dressing in which accelerating wound closure and freshly healed tissue with high similarity to the normal skin was reorganized. © 2021 Wiley Periodicals LLC.