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Encapsulation of Curcumin Loaded Chitosan Nanoparticle Within Poly (Ε-Caprolactone) and Gelatin Fiber Mat for Wound Healing and Layered Dermal Reconstitution Publisher Pubmed



Zahiri M1, 2 ; Khanmohammadi M3 ; Goodarzi A3 ; Ababzadeh S4 ; Sagharjoghi Farahani M3 ; Mohandesnezhad S3 ; Bahrami N3 ; Nabipour I1 ; Ai J3, 5
Authors
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Authors Affiliations
  1. 1. The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
  2. 2. Department of Anatomical Sciences, School of Medical Sciences, Bushehr University of Medical Sciences, Bushehr, Iran
  3. 3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, 1417743361, Iran
  4. 4. Department of Tissue Engineering & Regenerative Medicine, Faculty of Medical Sciences, Qom University of Medical Sciences, Qom, Iran
  5. 5. Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: International Journal of Biological Macromolecules Published:2020


Abstract

Hybrid electrospun fiber containing bioactive molecules, which offer the ability to deliver the cells into the wound bed, will help to achieve a high therapeutic effect. In this study, an electrospun polycaperlactone (PCL) and gelatin (Gela) scaffold containing curcumin loaded chitosan nanoparticle (NCs/Cur) was used to evaluate in vivo wound healing ability of the fabricated scaffolds. The electrospun hybrid scaffold seeded with human endometrial stem cells (EnSCs) showed desirable biocompatibility with the host immune system and wound healing ability in a full-thickness excisional animal model. The constructs were characterized for structural, mechanical and biochemical properties. Fourier transform infrared spectroscopy (FTIR) confirmed all typical absorption characteristics of PCL and Gela polymers as well as NCs and Cur. The results showed the perfect contact angle, wettability and degradability of hybrid fiber scaffolds with the good mechanical and structural characteristics including shape uniformity, pore size and porosity. The cell attachment and proliferation on the PCL/Gela/NCs/Cur was higher than PCL and PCL/Gela scaffolds. In term of the capability of hybrid scaffold and EnSCs in histological analysis, this novel tissue-engineered construct could be suggested as a skin substitute to repair injured skin and regenerative medicine application. © 2019
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