Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! By
Evaluation of Nanonanoliposomal Curcumin on Cutaneous Leishmaniasis Skin Lesions Caused by Leishmania Major in Balb/C Mice Publisher



S Shirmohammad SEDIGHEH ; M Mohebali MEHDI ; F Arabkhazaeli FATEMEH ; J Hassan JALAL ; D Shayan DAVID ; N Amininia NARGES ; P Shayan PARVIZ
Authors

Source: Iranian Journal of Parasitology Published:2024


Abstract

Background: Curcumin is an extract of rhizome turmeric (diferuloylmethane), with antioxidant, anti-inflammatory, antimicrobial, and anti-parasitic properties, which making it a potential candidate for the treatment of leishmaniasis. The aim of the presented study was to evaluate curcumin as possible candidate for treatment of cutaneous leishmaniasis. Methods: We investigated the physicochemical properties and anti-leishmanial effects of nanoliposomal curcumin (40, 80, and 120 μM) in Leishmania major (MRHO/IR/75/ER) infected BALB/c mice at the faculty of Veterinary Medicinem University of Tehran, Iran. For this aim, L. major promastigotes (MHROM/IR/75/ER) at stationary phase (2×106) were inoculated sub-cutaneously into the upper area of the tail in BALB/c mice (six groups, n= 10 per group). For evaluation of nanoliposomal curcumin, the zeta potential, particle size and stability of nanoliposomal curcumin was determined. Furthermore, the antileishmanial effects of nanoliposomal curcumin formulation on the lesion sizes was determined and the parasite burden in the leishmania induced lesion was per-formed using semi quantitative PCR. Results: Treatment of L. major infected BALB/c mice with nanoliposomal curcumin led to a reduction in the kinetic of the skin lesion size development. The semi quantitative PCR analysis of DNA extracted from the lesions showed reduction of parasite burden. The most effective treatment could be found in 80 μM nanoliposomal curcumin. Treatment with Glucantime, as a positive control, also showed a nearly similar effect compared to the effect of 80 μM nanoliposomal curcumin. Conclusion: Nanoliposomal curcumin could be considered as a potential drug against cutaneous leishmaniasis caused by L. major in susceptible animal models. © 2024 Elsevier B.V., All rights reserved.
Related Docs
View other Related Docs
1. Evaluation of Curcumin and Cm11 Peptide Alone and in Combination Against Amastigote Form of Iranian Strain of L. Major (Mrho/Ir75/Er) in Vitro, Experimental Parasitology (2021)
2. Plga Nanoparticles As New Drug Delivery Systems in Leishmaniasis Chemotherapy: A Review of Current Practices, Current Medicinal Chemistry (2024)
3. Determination of the Effective Dose of Curcumin Alone and in Combination With Antimicrobial Peptide Cm11 on Promastigote Forms of Iranian Strain of L. Major (Mrho / Ir / 75 / Er); [Determination De La Dose Efficace De Curcumine Seule Et En Association Avec Le Peptide Antimicrobien Cm11 Sur La Forme Promastigote De La Souche Iranienne Leishmania Major (Mrho/Ir/75/Er)], Archives of Razi Institute (2019)
Experts (# of related papers)
View all Related Experts
Alireza Firooz (2)
Mahdi Mohebali (2)
Other Related Docs
4. The Role of Atp-Binding Cassette Transporter Genes Expression in Treatment Failure Cutaneous Leishmaniasis, AMB Express (2022)
5. Evaluation of Expression Variations in Virulence-Related Genes of Leishmania Major After Several Culture Passages Compared With Phlebotomus Papatasi Isolated Promastigotes, PLoS ONE (2023)
6. The Effects of Medicinal Herbs and Marine Natural Products on Wound Healing of Cutaneous Leishmaniasis: A Systematic Review, Microbial Pathogenesis (2021)
7. Biophysical and Ultrasonographic Changes of Acute Old World Cutaneous Leishmaniasis Skin Lesions in Comparison With Uninvolved Skin: A Possible Tool for Non-Invasive Early Detection and Treatment Outcome Assessment, Dermatologic Therapy (2022)
8. The Therapeutic Effects of Olive Leaf Extract on Leishmania Major Infection in Balb/C Mice, Marmara Pharmaceutical Journal (2017)
9. The Role of Mpl and Imiquimod Adjuvants in Enhancement of Immune Response and Protection in Balb/C Mice Immunized With Soluble Leishmania Antigen (Sla) Encapsulated in Nanoliposome, Artificial Cells# Nanomedicine and Biotechnology (2018)
10. Induction of Immune Responses by Dna Vaccines Formulated With Dendrimer and Poly (Methyl Methacrylate) (Pmma) Nano-Adjuvants in Balb/C Mice Infected With Leishmania Major, Open Access Macedonian Journal of Medical Sciences (2018)
11. Multi-Antigen Vaccination With Lpd Nanoparticles Containing Rgp63 and Rlmac1n Proteins Induced Effective Immune Response Against Leishmaniasis in Animal Model, Journal of Drug Delivery Science and Technology (2021)
12. Effect of Peel and Leaf Extract of Walnut (Juglans Regia L.) on Cutaneous Leishmaniasis Caused by Leishmania Major in Balb/C Mice, Journal of Chemical and Pharmaceutical Sciences (2016)
13. Harmonized Clinical Trial Methodologies for Localized Cutaneous Leishmaniasis and Potential for Extensive Network With Capacities for Clinical Evaluation, PLoS Neglected Tropical Diseases (2018)
14. Niosomal Formulation of Amphotericin B Alone and in Combination With Glucantime: In Vitro and in Vivo Leishmanicidal Effects, Biomedicine and Pharmacotherapy (2019)
15. Combination of Topical Liposomal Amphotericin B and Glucantime in Comparison With Glucantime Alone for the Treatment of Anthroponotic Cutaneous Leishmaniasis (Acl) Caused by Leishmania Tropica: Study Protocol for a Randomized, Controlled Trial, Iranian Journal of Microbiology (2021)
16. In Vitro and in Vivo Therapeutic Potentials of 6-Gingerol in Combination With Amphotericin B for Treatment of Leishmania Major Infection: Powerful Synergistic and Multifunctional Effects, International Immunopharmacology (2021)
17. Anti-Leishmanial Effects of Resveratrol and Resveratrol Nanoemulsion on Leishmania Major, BMC Microbiology (2022)
18. Evaluation of a New Topical Treatment for the Control of Cutaneous Leishmaniasis, Microorganisms (2020)
19. In Vitro and in Vivo Anti-Parasitic Activity of Artemisinin Combined With Glucantime and Shark Cartilage Extract on Iranian Strain of Leishmania Major (Mrho/Ir/75/Er), Jundishapur Journal of Microbiology (2021)
20. Novel Strategies and Pharmaceutical Agents for the Treatment of Leish-Maniasis: A Review, Anti-Infective Agents (2020)