Whole Cell Fret Immunosensor Based on Graphene Oxide and Graphene Dot for Campylobacter Jejuni Detection

Whole Cell Fret Immunosensor Based on Graphene Oxide and Graphene Dot for Campylobacter Jejuni Detection Publisher Pubmed

Dehghani Z¹ ; Mohammadnejad J¹ ; Hosseini M^{1, 2} ; Bakhshi B³ ; Rezayan AH¹

Show Affiliations

1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran
2. Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center (MBRC), Faculty of Pharmacy, Tehran Univercity of Medical Sciences, Tehran, Iran
3. Department of Bacteriology, Faculty of Medical Sciences, University of Tarbiat Modares, Tehran, Iran

Source: Food Chemistry Published:2020

Abstract

In this work a new fluorescence immunosensor with use of graphene oxide and graphene quantum dot for detection Campylobacter jejuni whole cell in food samples was designed. This biosensor was designed based on interaction of poly clonal antibody conjugated with graphene quantum dot with surface protein in Campylobacter jejuni cell membrane. Specific binding of graphene quantum dot with Campylobacter jejuni membrane leads to generate a distance among graphene dot and graphene oxide and fluorescence is ON. In lack of Campylobacter jejuni or in existence of other bacterial cells, distance between of graphene dot and graphene oxide is very low and graphene quantum dot fluorescence emission was OFF. Experiment revealed that step by step increase in bacterial target cells caused to gradually increased fluorescence emission and this process was linear. Limit of detection for this bacterial sensor was 10 CFU/ml and ability of this FRET immunosensor for Campylobacter jejuni sensing in comparison with other bacterial cells was significant. Also, this method for monitoring Campylobacter jejuni in poultry liver was applied and results revealed that this immunosensor could be used for analysis bacterial cell in food samples. © 2019 Elsevier Ltd

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Experts (# of related papers)

Bahram Nikmanesh (1)

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Style	Citing Format
MLA	Dehghani Z, et al.. "Whole Cell Fret Immunosensor Based on Graphene Oxide and Graphene Dot for Campylobacter Jejuni Detection." Food Chemistry, vol. 309, no. , 2020, pp. -.
APA	Dehghani Z, Mohammadnejad J, Hosseini M, Bakhshi B, Rezayan AH (2020). Whole Cell Fret Immunosensor Based on Graphene Oxide and Graphene Dot for Campylobacter Jejuni Detection. Food Chemistry, 309(), -.
Chicago	Dehghani Z, Mohammadnejad J, Hosseini M, Bakhshi B, Rezayan AH. "Whole Cell Fret Immunosensor Based on Graphene Oxide and Graphene Dot for Campylobacter Jejuni Detection." Food Chemistry 309, no. (2020): -.
Harvard	Dehghani Z et al. (2020) 'Whole Cell Fret Immunosensor Based on Graphene Oxide and Graphene Dot for Campylobacter Jejuni Detection', Food Chemistry, 309(), pp. -.
Vancouver	Dehghani Z, Mohammadnejad J, Hosseini M, Bakhshi B, Rezayan AH. Whole Cell Fret Immunosensor Based on Graphene Oxide and Graphene Dot for Campylobacter Jejuni Detection. Food Chemistry. 2020;309():-.
BibTex	@article{ author = {Dehghani Z and Mohammadnejad J and Hosseini M and Bakhshi B and Rezayan AH}, title = {Whole Cell Fret Immunosensor Based on Graphene Oxide and Graphene Dot for Campylobacter Jejuni Detection}, journal = {Food Chemistry}, volume = {309}, number = {}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Dehghani Z AU - Mohammadnejad J AU - Hosseini M AU - Bakhshi B AU - Rezayan AH TI - Whole Cell Fret Immunosensor Based on Graphene Oxide and Graphene Dot for Campylobacter Jejuni Detection JO - Food Chemistry VL - 309 IS - SP - EP - PY - 2020 ER -

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