Study on the Interaction of the Cpg Alternating Dna With Cdte Quantum Dots Publisher Pubmed



Hosseini M¹ ; Khaki F¹ ; Shokri E¹ ; Khabbaz H¹ ; Dadmehr M² ; Ganjali MR^{3, 4} ; Feizabadi M⁵ ; Ajloo D⁵ Show Affiliations
Source: Journal of Fluorescence Published:2017

Abstract

A novel sensitive method for detection of DNA methylation was developed with thioglycollic acid (TGA)-capped CdTe quantum dots (QDs) as fluorescence probes. Recognition of methylated DNA sites would be useful strategy due to the important roles of methylation in disease occurrence and developmental processes. DNA methylation occurs most often at cytosine-guanine sites (CpG dinucleotides) of gene promoters. The QDs significantly interacted with hybridized unmethylated and methylated DNA. The interaction of CpG rich methylated and unmethylated DNA hybrid with quantum dots as an optical probe has been investigated by fluorescence spectroscopy and electrophoresis assay. The fluorescence intensity of QDs was highly dependent to unmethylated and methylated DNA. Specific site of CpG islands of Adenomatous polyposis coli (APC), a well-studied tumor suppressor gene, was used as the detection target. Under optimum conditions, upon the addition of unmethylated dsDNA, the fluorescence intensity increased in linear range from 1.0 × 10− 10 to 1.0 × 10− 6M with detection limit of 6.2 × 10− 11 M and on the other hand, the intensity of QDs showed no changes with addition of methylated dsDNA. We also demonstrated that the unmethylated and methylated DNA and QDs complexes showed different mobility in electrophoresis assay. This easy and reliable method could distinguish between methylated and unmethylated DNA sequences. © 2017, Springer Science+Business Media, LLC.