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A Fluorometric Study on the Effect of Dna Methylation on Dna Interaction With Graphene Quantum Dots Publisher Pubmed



Rafiei S1 ; Dadmehr M2 ; Hosseini M1, 3 ; Kermani HA1 ; Ganjali MR4, 5
Authors
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Authors Affiliations
  1. 1. Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Post Code: 1417466191, Tehran, Iran
  2. 2. Department of Biology, Payame Noor University, Post Code: 1455643183, Tehran, Iran
  3. 3. Medical Biomaterials Research Center, Tehran University of Medical Sciences, Post Code: 1416753955, Tehran, Iran
  4. 4. Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Post Code: 1417466191, Tehran, Iran
  5. 5. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Post Code: 1416753955, Tehran, Iran

Source: Methods and Applications in Fluorescence Published:2019


Abstract

DNA methylation plays an important role in development process which contributes to genome stability and also regulates gene expression and gene silencing. Detection of genome regions with altered 5-methylcytosine distribution at a genome-wide scale is very important for early detection of gene silencing related diseases. In the present study as a continuation of studies on DNA methylation, the interactions between graphene quantum dots (GQDs) and unmethylated and methylated deoxyribonucleic acid (DNA) fragment were investigated. Based on above interaction a novel GQDs-DNA nanoassembly was developed. Two types of DNA including unmethylated and methylated sequences were interacted with GQDs and contributed to the formation of unmethylated and methylated nanoassemlies. Analysis of the interaction indicated that the GQDs could bind to DNA fragments and led to different fluorescence pattern in two different mechanisms and could provide an efficient biosensing platform for label free and sensitive fluorescent assay of DNA. The excitation and emission wavelengths of experiment were 380 and 480 nm respectively. Fluorescence intensity of unmethylated DNA concentration were detectable from methylated DNA in linear range from 10.0-10M to 10.0-6M and the detection limit was estimated at 7.3 10-11 M. Above interaction was not observed in methylated DNA, indicated of distinguished interaction effect. Herein we further showed that GQDs could induce B-DNA to A-DNA form in methylated structure of DNA. The methylation sensitivity of the experiment was also testified by methylation sensitive restriction process. It was assumed that the involvement of methylation alteration in DNA structure could alter not only mechanism of DNA/GDQs interaction but also helical structure of DNA. © 2019 IOP Publishing Ltd.
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