The Role of Inflammatory Mirna–Mrna Interactions in Pbmcs of Colorectal Cancer and Obesity Patients Publisher Pubmed



Gholami M^{1, 2} ; Zoughi M¹ ; Larijani B³ ; Abdollahzadeh R⁴ ; Taslimi R⁵ ; Rahmani Z⁵ ; Kazemeini A⁶ ; Behboo R⁷ ; Razi F⁸ ; Bastami M⁹ ; Hasaniranjbar S² ; Amoli MM¹ Show Affiliations
Source: Immunity# Inflammation and Disease Published:2022

Abstract

Introduction: Inflammation is a critical hallmark in obesity and colorectal cancer (CRC). This study aimed to investigate effective microRNA (miRNA)–messenger RNA (mRNA) interactions on inflammatory networks involved in obesity and CRC. Methods: The literature searches were applied to identify genes expression reported on peripheral blood mononuclear cells (PBMCs) and/or blood of CRC subjects and to find inflammatory miRNA in blood samples. Furthermore, bioinformatics analysis was utilized to find inflammatory miRNA:mRNA interactions of the genes. Finally, a case-control study was set to investigate the expression of LAMC1 and GNB3 genes besides miR-10b, miR-506-3p, miR-150-5p, and miR-124-3p in CRC and control subjects. Results: The expression of LAMC1 gene in healthy control groups was associated with body mass index (BMI) (p <.05). The level of miR-10b (p <.001), miR-506 (p <.001), and miR-124 (p <. 001) were significantly increased in PBMCs of CRC patients, while they were not associated with BMI. The level of miR-150 was associated with BMI in healthy subjects (p <.05). Conclusions: The changes in the level of miR-506 and miR-124 in CRC patients may be associated with the regulatory role of these miRNAs on LAMC1 expression. The LAMC1 may be related to BMI, however, more observational studies on other populations are needed. © 2022 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd.