Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Cytotoxicity Assessment of Polyhydroxybutyrate/Chitosan/Nano- Bioglass Nanofiber Scaffolds by Stem Cells From Human Exfoliated Deciduous Teeth Stem Cells From Dental Pulp of Exfoliated Deciduous Tooth Publisher



Hashemibeni B1 ; Khoroushi M2 ; Foroughi MR3 ; Karbasi S4 ; Khademi AA5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Dental Research Center and Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Dental Materials Research Center and Department of Operative Dentistry, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Dental Materials Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Biomaterials and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. Dental Research Center and Department of Endodontic, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Dental Research Journal Published:2018


Abstract

Background: The aim of this study was to compare the cytotoxicity and the biocompatibility of three different nanofibers scaffolds after seeding of stem cells harvested from human deciduous dental pulp. Given the importance of scaffold and its features in tissue engineering, this study demonstrated the construction of polyhydroxybutyrate (PHB)/chitosan/nano-bioglass (nBG) nanocomposite scaffold using electrospinning method. Materials and Methods: This experimental study was conducted on normal exfoliated deciduous incisors obtained from 6-year-old to 11-year-old healthy children. The dental pulp was extracted from primary incisor teeth which are falling aseptically. After digesting the tissue with 4 mg/ml of type I collagenase, the cells were cultured in medium solution. Identification of stem cells from human exfoliated deciduous teeth was performed by flowcytometry using CD19, CD14, CD146, and CD90 markers. Then, 1 × 104 stem cells were seeded on the scaffold with a diameter of 10 mm × 0.3 mm. Cell viability was evaluated on days 3, 5, and 7 through methyl thiazol tetrazolium techniques (P < 0.05) on different groups that they are groups included (1) PHB scaffold (G1), (2) PHB/chitosan scaffold (G2), (3) the optimal PHB/chitosan/nBG scaffold (G3), (4) mineral trioxide aggregate (MTA), and (5) the G3 + MTA scaffold (G3 + MTA). Data were analyzed with two-way ANOVA at significance level of P < 0.05. Results: The results indicated that the PHB/chitosan/nBG scaffold and PHB/chitosan/nBG scaffold + MTA groups showed significant difference compared with the PHB/chitosan scaffold and PHB scaffold groups on the 7th day (P < 0.05). Conclusion: Thus, it can be concluded that the scaffold with nBG nanoparticles is more biocompatible than the other scaffolds and can be considered as a suitable scaffold for growth and proliferation of stem cells. © 2018 Dental Research Journal | Published by Wolters Kluwer - Medknow.
Related Docs
View other Related Docs
1. Effect of Polyhydroxybutyrate/Chitosan/Bioglass Nanofiber Scaffold on Proliferation and Differentiation of Stem Cells From Human Exfoliated Deciduous Teeth Into Odontoblast-Like Cells, Materials Science and Engineering C (2018)
2. Tissue Engineering: Dentin – Pulp Complex Regeneration Approaches (A Review), Tissue and Cell (2017)
3. Bone Tissue Engineering Using Human Cells: A Comprehensive Review on Recent Trends, Current Prospects, and Recommendations, Applied Sciences (Switzerland) (2019)
4. An Improved Surface for Enhanced Stem Cell Proliferation and Osteogenic Differentiation Using Electrospun Composite Plla/P123 Scaffold, Artificial Cells# Nanomedicine and Biotechnology (2018)
5. Biological Evaluation of the Effects of Hyaluronic Acid on Poly (3-Hydroxybutyrate) Based Electrospun Nanocomposite Scaffolds for Cartilage Tissue Engineering Application, Materials Technology (2020)
6. Mesenchymal Stromal Cells; a New Horizon in Regenerative Medicine, Journal of Cellular Physiology (2020)
7. Mesenchymal Stem Cells in the Treatment of Amyotrophic Lateral Sclerosis, Current Stem Cell Research and Therapy (2016)
8. Chitosan-Encapsulated Lipid-Based Nanovesicles for Therapeutic Applications and Tissue Engineering: A Review, Carbohydrate Polymer Technologies and Applications (2025)
Experts (# of related papers)
View all Related Experts
Seyed Mahdi Rezayat Sorkhabadi (3)
Hamidreza Aghayan (2)
Other Related Docs
9. In Vitro Investigation of Canine Periodontal Ligament-Derived Mesenchymal Stem Cells: A Possibility of Promising Tool for Periodontal Regeneration, Journal of Oral Biology and Craniofacial Research (2023)
10. Reinforcing Mechanical Strength of Electrospun Chitosan Nanofibrous Scaffold Using Cellulose Nanofibers, Journal of Nano Research (2018)
11. Multipotent Stem Cell and Current Application, Acta Medica Iranica (2017)
12. Evaluation of the Effects of Keratin on Physical, Mechanical and Biological Properties of Poly (3-Hydroxybutyrate) Electrospun Scaffold: Potential Application in Bone Tissue Engineering, European Polymer Journal (2020)
13. Adipose Tissue-Derived Stromal Cells for Wound Healing, Advances in Experimental Medicine and Biology (2018)
14. Concomitant Transurethral and Transvaginal-Periurethral Injection of Autologous Adipose Derived Stem Cells for Treatment of Female Stress Urinary Incontinence: A Phase One Clinical Trial, Acta Medica Iranica (2017)
15. Mesenchymal Stromal Cell-Based Novel Treatment Modalities in Rheumatoid Arthritis, Handbook of Stem Cell Applications (2024)
16. Evaluation and Comparison of the in Vitro Characteristics and Chondrogenic Capacity of Four Adult Stem/Progenitor Cells for Cartilage Cell-Based Repair, Journal of Biomedical Materials Research - Part A (2016)
17. Combined Effect of Retinoic Acid and Calcium on the in Vitro Differentiation of Human Adipose-Derived Stem Cells to Adipocytes, Archives of Physiology and Biochemistry (2018)
18. Literature Review of Adipose-Derived Mesanchymal Cells From History to Approaches, Iranian Red Crescent Medical Journal (2017)
19. Mesenchymal Stem Cells: Rising Concerns Over Their Application in Treatment of Type One Diabetes Mellitus, Journal of Diabetes Research (2015)
20. Evaluation of the Effects of Hyaluronic Acid on Poly (3-Hydroxybutyrate)/Chitosan/Carbon Nanotubes Electrospun Scaffold: Structure and Mechanical Properties, Polymer-Plastics Technology and Materials (2019)