Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Wnt Pathway Activator Delivery by Poly (Lactide-Co-Glycolide)/Silk Fibroin Composite Nanofibers Promotes Dental Pulp Stem Cell Osteogenesis Publisher



Sohrabi A1 ; Hosseini M2 ; Abazari MF3 ; Zare Karizi S4 ; Sadeghi Oskouei SA4 ; Hajatibirgani N5 ; Karimi Hafshejani F6 ; Hashemi SAR7 ; Rahmati M8 ; Askari M9
Authors
Show Affiliations
Authors Affiliations
  1. 1. School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran
  2. 2. Diagnostic and Therapeutic Industrial Group, Khayyam Innovation Ecosystem, Mashhad, Iran
  3. 3. Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Biology, Varamin Pishva Branch, Islamic Azad University, Pishva, Varamin, Iran
  5. 5. Department of Biology, Faculty of Science and Research, Islamic Azad University, Tehran, Iran
  6. 6. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  8. 8. Department of Medical Biotechnology, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran
  9. 9. National Cell Bank Department, Pasteur Institute of Iran, Tehran, Iran

Source: Journal of Drug Delivery Science and Technology Published:2021


Abstract

The high regenerative ability of damaged bone tissue in Oral and maxillofacial area has made it an optimal target for tissue engineering. Introducing scaffold that has high biocompatibility and good degradability and can also deliver biological factors well is the most important goal of tissue engineering. In the present study, poly(lactide-co-glycolide) (PLGA)/silk fibroin (SF) nanofibrous scaffolds with and without inorganic polyphosphate (polyP) as a Wnt pathway activator was fabricated via electrospinning. The potential application of fabricated scaffolds in oral and maxillofacial tissue engineering was investigated with the culture of isolated and characterized dental pulp stem cells (DPSCs) in vitro. The obtained results demonstrated that the highest viability of the DPSCs was detected when grown on the PLGA/SF/polyP nanofibers compared to the PLGA/SF nanofibers. Furthermore, DPSCs cultured on PLGA/SF/polyP nanofibers exhibited a strong osteogenic differentiation potential compared to when cultured on the PLGA/SF nanofibers or standard culture plate. Therefore, according to the results obtained in this study, the PLGA/SF/polyP nanofibrous scaffold in combination with DPSCs or alone has a great potential to use for bone reconstruction in oral and maxillofacial tissue engineering applications. © 2020 Elsevier B.V.
Related Docs
1. Cartilage and Facial Muscle Tissue Engineering and Regeneration: A Mini Review, Bio-Design and Manufacturing (2018)
2. Incorporated-Bfgf Polycaprolactone/Polyvinylidene Fluoride Nanocomposite Scaffold Promotes Human Induced Pluripotent Stem Cells Osteogenic Differentiation, Journal of Cellular Biochemistry (2019)
3. Cell Homing Strategy As a Promising Approach to the Vitality of Pulp-Dentin Complexes in Endodontic Therapy: Focus on Potential Biomaterials, Expert Opinion on Biological Therapy (2022)
4. Nanofiber-Based Systems Intended for Diabetes, Journal of Nanobiotechnology (2021)
5. Recent Approaches to Enhance Osteogenesis of Dental Pulp Stem Cells on Electrospun Scaffolds, Current Stem Cell Research and Therapy (2024)
6. Application of Biocompatible Scaffolds in Stem-Cell-Based Dental Tissue Engineering, Advances in Experimental Medicine and Biology (2023)
7. Synergistic Effects of Polyaniline and Pulsed Electromagnetic Field to Stem Cells Osteogenic Differentiation on Polyvinylidene Fluoride Scaffold, Artificial Cells# Nanomedicine and Biotechnology (2019)
Experts (# of related papers)
View all Related Experts
Farshad Sharifi (1)
Seyed Mahmoud Sajjadi Jazi (1)