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The Effects of Chlamydia Trachomatis, Mycoplasma Hominis, and Ureaplasma Urealyticum Loads on Semen Quality: Detection and Quantitative Analysis Publisher Pubmed



Dehghan A1 ; Pourmand MR1 ; Salimi V2 ; Asbagh FA3 ; Foroushani AR4 ; Sadeghi K2 ; Quchani SH5
Authors
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Authors Affiliations
  1. 1. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Obstetrics and Gynecology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Yas Hospital Infertility Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Microbial Pathogenesis Published:2022


Abstract

Background: The loads of Chlamydia trachomatis (CT), Mycoplasma hominis (MH), and Ureaplasma urealyticum (UU) may impact infertility, as well as cause risk of transmission. The quality and quantity of semen demonstrate male reproductive health. This study aimed to investigate the semen quality affected by CT, MH, and UU loads. Materials and methods: 130 semen samples, including infertile and fertile cases, were collected and analyzed. The whole genomic DNA was extracted, and the desired genes' plasmids were constructed. The CT, MH, and UU loads were quantified by real-time PCR. The data were analyzed using SPSS version 24. Results: The average age of participants was 35.2 ± 6.8 years. CT, MH, and UU frequency were 9.2% vs. 3.1%, 15.4% vs. 3.1%, and 15.4 vs. 3.1% in infertile and fertile men, respectively. The mean loads of CT, MH, and UU in infertile men were 6.44 log10 copies/ml (range 5.31–7), 4.24 log10 copies/ml (range 3.37–4.7), and 6.94 log10 copies/ml (range 5.08–8.69) respectively, which was significantly higher than fertile men. The findings revealed a significant correlation between CT and UU loads and semen parameters, whereas the load of MH displayed significant effects just on sperm motility, morphology, and the number of leukocytes. Conclusion: The absence of clinical manifestations may not indicate the quality of semen. The pathogens' loads may significantly influence the quality and properties of male reproductive health. © 2022 Elsevier Ltd
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