Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Correlation Between Ros Generation and Lpo Process As the Function of Methylparaben Concentrations During Hemoglobin Fructation Publisher



Pishkari N1 ; Habibirezaei M2, 3 ; Taghavi F4, 8 ; Amanlou M5 ; Sheibani N6 ; Saso L7 ; Moosavimovahedi AA1, 8
Authors
Show Affiliations
Authors Affiliations
  1. 1. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
  2. 2. School of Biology, College of Science, University of Tehran, Tehran, Iran
  3. 3. Center of Excellence in NanoBiomedicine, University of Tehran, Tehran, Iran
  4. 4. Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran
  5. 5. Department of Medicinal Chemistry, Faculty of Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Ophthalmology and Visual Sciences, Cell and Regenerative Biology, and Biomedical Engineering, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States
  7. 7. Department of Physiology and Pharmacology “Vittorio Erspamer�, Sapienza University of Rome, P. le Aldo Moro 5, Rome, 00185, Italy
  8. 8. UNESCO Chair on Interdisciplinary Research in Diabetes, University of Tehran, Tehran, Iran

Source: Journal of the Iranian Chemical Society Published:2020


Abstract

In this study, we focused on the interference of methylparaben (MP), as the most commonly used paraben, on hemoglobin (Hb) fructation, generating reactive oxygen species (ROS) and resulting lipid peroxidation (LPO). We showed the enhancement of ROS generation and heme degradation during hemoglobin fructation in the presence of MP using some biophysical techniques such as chemiluminescence and fluorescence spectroscopies, respectively. Lipid peroxidation was observed using thiobarbituric acid reactive substances (TBARS) assay, which correlated with ROS production. Also, the results showed that by increasing the levels of fructation, especially in diabetic-like groups, ROS production was increased augmenting the intensity of LPO. This was accompanied by the elevation of malondialdehyde (MDA) production. Thus, MP has an adverse impact on hemoglobin by enhancing advance glycation end products (AGEs) and ROS generation upon its fructation, which results in MDA generation through enhanced LPO. This kind of research emphasises the importance of preservatives’ application revision especially methylparaben in various industries and the necessity of special attention to the consumption of these products by diabetic patients. © 2020, Iranian Chemical Society.
Related Docs
1. Antiamyloidogenic Effects of Ellagic Acid on Human Serum Albumin Fibril Formation Induced by Potassium Sorbate and Glucose, Journal of Molecular Recognition (2016)
Experts (# of related papers)
Massoud Amanlou (1)