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Synergistic Induction of Cardiomyocyte Differentiation From Human Bone Marrow Mesenchymal Stem Cells by Interleukin 1Β and 5-Azacytidine Publisher Pubmed



Khajeniazi S1, 2 ; Solati M2 ; Yazdani Y3 ; Soleimani M4 ; Kianmehr A2, 5
Authors
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Authors Affiliations
  1. 1. Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran
  2. 2. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, 49189-36316, Iran
  3. 3. Infectious Diseases Research Center, Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, 49189-36316, Iran
  4. 4. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, 14115-111, Iran
  5. 5. Biochemistry and Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, 49189-36316, Iran

Source: Biological Chemistry Published:2016


Abstract

Interleukin-1β (IL-1β) is a cytokine protein expressed by cardiomyocyte in myocardial damage-associated inflammation. Although several methods are currently available for treatment of heart diseases none of them are completely successful. Recently, stem cells have gained enormous attention and are expected to play a significant role for treating heart diseases. 5-Azacytidine (5-aza) has recently been found to cause stimulation of stem cells to differentiate into cardiomyocytes. Here we present the determination of whether IL-1β can induce the differentiation of mesenchymal stem cells (MSCs) to cardiomyocytes. MSCs were derived from bone marrow, propagated and then cultured in differentiation medium supplemented with 5-aza and IL-1β (at two levels, 5 and 10 ng/ml). After 21 days, total RNA was extracted and cDNA synthesis was carried out. Quantitative polymerase chain reaction (Q-PCR) was performed for detecting cardiac-specific markers. Besides, to investigate the expression of cardiac markers in protein levels, immunocytochemistry was done by specific antibodies. Ultimately, cardiac markers expression suggested that IL-1β and 5-aza synergistically induces the cardiomyocyte differentiation. © 2016 Walter de Gruyter GmbH, Berlin/Boston.